Zillmann U, Albiez E J
Trop Med Parasitol. 1986 Dec;37(4):390-2.
The inhabitants of three Liberian villages in a known gambiense sleeping sickness area with a low prevalence were examined comparatively with the haematocrit centrifugation technique (HCT), the miniature anion exchange centrifugation technique (m-AECT), the indirect fluorescent antibody test (IFAT) and the Testryp CATT (Card Agglutination Test for Trypanosomiasis). Among 418 persons examined was one individual from whom trypanosomes could be isolated with the HCT and m-AECT. This positive case and another 21 individuals had positive titres of 1:40 and more in the IFAT. The same number, but not the same individuals, showed positive reactions in the CATT in the 1:5 serum dilution. The 1:25 dilution of blood and serum was inferior to the corresponding 1:5 dilution and could therefore be omitted. The 'false positive' rate in the CATT was 5.0% in the serum, 10.1% in the blood, and 11.5% in blood or serum. The combination of HCT and m-AECT in the field did not achieve better results than the CATT or IFAT. Thus the CATT can be recommended as the preferable technique for screening of gambiense trypanosomiasis in the field.
在一个已知的冈比亚锥虫病低流行区，对三个利比里亚村庄的居民采用血细胞比容离心技术（HCT）、微型阴离子交换离心技术（m - AECT）、间接荧光抗体试验（IFAT）和锥虫病卡片凝集试验（Testryp CATT）进行了比较检查。在接受检查的418人中，有一人通过HCT和m - AECT分离出了锥虫。该阳性病例以及另外21人在IFAT中的滴度为1:40及以上呈阳性。同样数量（但不是相同个体）在CATT的1:5血清稀释度下呈阳性反应。血液和血清的1:25稀释度不如相应的1:5稀释度，因此可以省略。CATT在血清中的“假阳性”率为5.0%，在血液中为10.1%，在血液或血清中为11.5%。在现场，HCT和m - AECT联合使用的效果并不比CATT或IFAT更好。因此，CATT可被推荐为现场筛查冈比亚锥虫病的首选技术。
