Matinhira Naboth, Soko Nyarai D, Bandason Tsitsi, Jenson Ramon G, Dzongodza Titus, von Buchwald Christian, Chidziva Clemence
Harare Eye, Ear, Nose and Throat Institute, Milton Park, Harare, Zimbabwe; Department of Surgery, Faculty of Medicine, University of Zimbabwe, Harare, Zimbabwe.
Harare Eye, Ear, Nose and Throat Institute, Milton Park, Harare, Zimbabwe.
Int J Pediatr Otorhinolaryngol. 2019 Jan;116:147-152. doi: 10.1016/j.ijporl.2018.10.036. Epub 2018 Oct 26.
Recurrent respiratory papillomatosis (RRP) caused by human papillomavirus (HPV) is preventable through vaccination. This study was motivated by the recent thrust of the Zimbabwean government to reduce incidence of HPV related cervical cancer in Zimbabwe through vaccination against HPV. We therefore set out to type HPV genotypes causing RRP in Zimbabwe. We also describe for the first time, the demographics of Zimbabwean RRP patients, the characteristics of patients with different HPV types and possible risk factors of HPV infection in our setting.
We conducted a prospective, hospital based study were patients were recruited from two national otorhinolaryngology hospitals in Zimbabwe. All patients diagnosed with RRP during a twenty four month period were included in the study. A questionnaire was administered per patient to collect both demographic and clinical variables. HPV DNA was extracted from formalin fixed paraffin embedded laryngeal tissue. The extracted HPV DNA was amplified using polymerase chain reaction and next generation sequencing was used to genotype the HPV types.
A total of 52 patients all aged 14 years and under were recruited into the study. Only Juvenile onset RRP cases were observed over the two year period and 64% of the patients were HPV positive. HPV types 6 and 11 were the dominant types observed constituting 85% of all HPV types. The remaining 15% constituted of HPV 16 and HPV 18. 27% of the patients had coinfection with at least two different HPV types. There were no statistically significant differences between the characteristics of HPV positive and HPV negative patients. No statistically significant risk factors were observed.
HPV types 6 and 11 were the predominant genotypes causing RRP in Zimbabwe. Thus the use of quadrivalent or even nonavalent HPV vaccines may play an important role in the prevention and management of RRP in Zimbabwe.
人乳头瘤病毒（HPV）引起的复发性呼吸道乳头状瘤病（RRP）可通过接种疫苗预防。本研究的动机是津巴布韦政府最近为通过接种HPV疫苗降低津巴布韦HPV相关宫颈癌发病率所做的努力。因此，我们着手对导致津巴布韦RRP的HPV基因型进行分型。我们还首次描述了津巴布韦RRP患者的人口统计学特征、不同HPV类型患者的特点以及我们研究环境中HPV感染的可能危险因素。
我们进行了一项基于医院的前瞻性研究，从津巴布韦的两家国立耳鼻喉科医院招募患者。在24个月期间所有被诊断为RRP的患者都纳入了研究。对每位患者进行问卷调查以收集人口统计学和临床变量。从福尔马林固定石蜡包埋的喉组织中提取HPV DNA。使用聚合酶链反应扩增提取的HPV DNA，并使用下一代测序对HPV类型进行基因分型。
共有52名年龄均在14岁及以下的患者被纳入研究。在这两年期间仅观察到青少年期发病的RRP病例，64%的患者HPV呈阳性。HPV 6型和11型是观察到的主要类型，占所有HPV类型的85%。其余15%由HPV 16型和HPV 18型组成。27%的患者至少感染了两种不同的HPV类型。HPV阳性和HPV阴性患者的特征之间没有统计学上的显著差异。未观察到统计学上显著的危险因素。
HPV 6型和11型是导致津巴布韦RRP的主要基因型。因此，使用四价甚至九价HPV疫苗可能在津巴布韦RRP的预防和管理中发挥重要作用。
