Periasamy Srinivasan, Lin Chia-Hui, Nagarajan Balaji, Sankaranarayanan Nehru Viji, Desai Umesh R, Liu Ming-Yie
Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 70428, Taiwan.
Institute for Structural Biology, Drug Discovery and Development and Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, United States of America.
J Funct Foods. 2018 Aug;47:1-10. doi: 10.1016/j.jff.2018.05.035. Epub 2018 May 24.
Tamarind xyloglucan (TXG) is edible, bioavailable and mucoadhesive polysaccharide. The aim of this study was (i) to investigate molecular docking studies on the interaction of TXG to MUC1 and cytokine receptors and (ii) to assess the mucoadhesive role of TXG in UC. study: C57Bl6 mice were administered with DSS 3% (w/v) in drinking water; TXG 100 or 300 mg/kg/day was given orally for 7 days simultaneously. TXG consistently binds to MUC1 and cytokine receptors in molecular docking studies. TXG decreased the expression of MUC1 and MUC2. The mucoadhesive ability of TXG decreased IL-1β and IL-6 levels. Furthermore, TXG decreased the expression of TLR4, MyD88, I-κB and NF-κB thereby attenuating inflammation via TLR4/NF-κB signaling pathway. TXG mucoadhesion to MUC1 played a pivotal role in attenuating inflammation. To conclude, the mucoadhesive role of TXG is important in the attenuation of inflammation and healing of UC.
罗望子木葡聚糖（TXG）是一种可食用、可生物利用且具有黏膜黏附性的多糖。本研究的目的是：（i）研究TXG与MUC1和细胞因子受体相互作用的分子对接研究；（ii）评估TXG在溃疡性结肠炎（UC）中的黏膜黏附作用。研究方法：给C57Bl6小鼠饮用含3%（w/v）葡聚糖硫酸钠（DSS）的水；同时每天口服100或300mg/kg的TXG，持续7天。在分子对接研究中，TXG始终与MUC1和细胞因子受体结合。TXG降低了MUC1和MUC2的表达。TXG的黏膜黏附能力降低了白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）水平。此外，TXG降低了Toll样受体4（TLR4）、髓样分化因子88（MyD88）、核因子κB抑制蛋白（I-κB）和核因子κB（NF-κB）的表达，从而通过TLR4/NF-κB信号通路减轻炎症。TXG与MUC1的黏膜黏附在减轻炎症中起关键作用。总之，TXG的黏膜黏附作用在减轻UC炎症和愈合中很重要。
