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硒代蛋氨酸对大鼠辐射诱导的急性肺炎和肺纤维化的保护作用

Protective Effect of Selenium-L-methionine on Radiation-induced Acute Pneumonitis and Lung Fibrosis in Rat.

作者信息

Amini Peyman, Kolivand Sedighe, Saffar Hana, Rezapoor Saeed, Motevaseli Elahe, Najafi Masoud, Nouruzi Farzad, Shabeeb Dheyauldeen, Musa Ahmed Eleojo

机构信息

Department of Radiology, Faculty of Paramedical, Tehran University of Medical Sciences, Tehran, Iran.

Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Curr Clin Pharmacol. 2019;14(2):157-164. doi: 10.2174/1574884714666181214101917.

Abstract

BACKGROUND

In this study, we aimed to detect the changes in the level of interleukin (IL)-4 and IL-13 cytokines and their downstream genes including interleukin-13 receptor subunit alpha-2 (IL13Ra2), interleukin-4 receptor subunit alpha-1 (IL4Ra1), dual oxidase 1 (DUOX1) and dual oxidase 2 (DUOX2). The protective effects of Selenium-L-methionine on radiation-induced histopathological damages and changes in the level of these cytokines and genes were detected.

METHODS

Four groups of 20 rats (5 rats in each) namely, control; Selenium-L-methionine, radiation and radiation plus Selenium-L-methionine were used in this study. 4 mg/kg of Selenium-Lmethionine was administered 1 day before irradiation and five consecutive days after irradiation. Irradiation was done using a dose of 15 Gy 60Co gamma rays at 109 cGy/min. All rats were sacrificed 10 weeks after irradiation for detecting changes in IL-4 and IL-13 cytokines, the expressions of IL13Ra2, IL4Ra1, Duox1 and Duox2 and histopathological changes.

RESULTS

The level of IL-4 but not IL-13 increased after irradiation. This was associated with increased expression of IL4Ra1, Duox1 and Duox2, in addition to changes in morphological properties. Selenium-L-methionine could attenuate all injury markers following lung irradiation.

CONCLUSION

Selenium-L-methionine can protect lung tissues against toxic effects of ionizing radiation. It is possible that the modulation of immune responses and redox interactions are involved in the radioprotective effect of this agent.

摘要

背景

在本研究中,我们旨在检测白细胞介素(IL)-4和IL-13细胞因子及其下游基因的水平变化,这些下游基因包括白细胞介素-13受体亚基α-2(IL13Ra2)、白细胞介素-4受体亚基α-1(IL4Ra1)、双氧化酶1(DUOX1)和双氧化酶2(DUOX2)。检测了硒代蛋氨酸对辐射诱导的组织病理学损伤以及这些细胞因子和基因水平变化的保护作用。

方法

本研究使用四组20只大鼠(每组5只),即对照组、硒代蛋氨酸组、辐射组和辐射加硒代蛋氨酸组。在照射前1天及照射后连续5天给予4mg/kg的硒代蛋氨酸。使用剂量为15Gy的60Coγ射线,以109cGy/min的速度进行照射。照射后10周处死所有大鼠,以检测IL-4和IL-13细胞因子的变化、IL13Ra2、IL4Ra1、Duox1和Duox2的表达以及组织病理学变化。

结果

照射后IL-4水平升高,而IL-13水平未升高。这与IL4Ra1、Duox1和Duox2的表达增加以及形态学特性的变化有关。硒代蛋氨酸可减轻肺部照射后的所有损伤标志物。

结论

硒代蛋氨酸可保护肺组织免受电离辐射的毒性作用。该药物的辐射防护作用可能涉及免疫反应和氧化还原相互作用的调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1768/7040518/5bddad1d00fd/CCP-14-157_F1.jpg

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