Department of General Surgery, the First People's Hospital of Wenling, Wenling, China.
Eur Rev Med Pharmacol Sci. 2018 Dec;22(23):8257-8264. doi: 10.26355/eurrev_201812_16520.
The aim of this study was to investigate the function of FAL1 in gastric cancer (GC) development and to examine its underlying mechanism. Our study might provide a theoretical basis for developing novel diagnostic markers for GC.
FAL1 expression in GC tissues and adjacent tissues was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The serum level of FAL1 in GC patients with different pathological grades was further detected. The effects of FAL1 on cell proliferation and cell cycle were detected by cell counting kit-8 (CCK-8) assay and flow cytometry, respectively. Meanwhile, Western blot was used to detect the protein expression of PTEN after FAL1 overexpression or knockdown in GC cells. In addition, rescue experiments were conducted to verify the regulatory effect of FAL1 on PTEN.
QRT-PCR results showed that the expression of FAL1 in GC tissues was remarkably higher than that of adjacent tissues. FAL1 expression was correlated with pathological grades of GC patients. Meanwhile, FAL1 overexpression promoted the proliferation and cell cycle of BGC-823 and MGC-803 cells. Western blot analysis demonstrated that FAL1 could inhibit the protein expression of PTEN in GC cells. In addition, rescue experiments indicated that the overexpression of PTEN could partially reverse the effect of FAL1 on the proliferation and cell cycle of BGC-823 and MGC-803 cells.
The overexpression of FAL1 can promote cell proliferation and cell cycle of GC via inhibiting PTEN.
本研究旨在探讨 FAL1 在胃癌（GC）发展中的作用，并探讨其潜在机制。我们的研究可能为开发 GC 的新型诊断标志物提供理论基础。
通过实时定量聚合酶链反应（qRT-PCR）检测 GC 组织和相邻组织中 FAL1 的表达。进一步检测不同病理分级 GC 患者血清中 FAL1 的水平。通过细胞计数试剂盒-8（CCK-8）测定和流式细胞术分别检测 FAL1 对细胞增殖和细胞周期的影响。同时，通过 Western blot 检测 GC 细胞中 FAL1 过表达或敲低后 PTEN 的蛋白表达。此外，进行了挽救实验以验证 FAL1 对 PTEN 的调节作用。
qRT-PCR 结果表明，GC 组织中 FAL1 的表达明显高于相邻组织。FAL1 的表达与 GC 患者的病理分级相关。同时，FAL1 的过表达促进 BGC-823 和 MGC-803 细胞的增殖和细胞周期。Western blot 分析表明，FAL1 可以抑制 GC 细胞中 PTEN 的蛋白表达。此外，挽救实验表明，PTEN 的过表达可以部分逆转 FAL1 对 BGC-823 和 MGC-803 细胞增殖和细胞周期的影响。
FAL1 的过表达可以通过抑制 PTEN 促进 GC 细胞的增殖和细胞周期。
