• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码 RNA FEZF1-AS1 通过调控 miR-363-3p/PAX6 轴促进视网膜母细胞瘤的生长并减少细胞凋亡。

Long Non-coding RNA FEZF1-AS1 Promotes Growth and Reduces Apoptosis Through Regulation of miR-363-3p/PAX6 Axis in Retinoblastoma.

机构信息

Department of Ophthalmology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, No. 1, Huanghe Road, Huaiyin District, Huaian, 223300, Jiangsu, China.

出版信息

Biochem Genet. 2021 Jun;59(3):637-651. doi: 10.1007/s10528-020-10026-7. Epub 2021 Jan 11.

DOI:10.1007/s10528-020-10026-7
PMID:33432525
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8096736/
Abstract

Retinoblastoma is the most common malignancy in children's eyes with high incidence. Long non-coding RNAs (lncRNAs) play important roles in the progression of retinoblastoma. LncRNA FEZF1 antisense RNA 1 (FEZF1-AS1) has been found to stimulate retinoblastoma. However, the mechanism of FEZF1-AS1 underlying progression of retinoblastoma is still unclear. In current study, FEZF1-AS1 was up-regulated in retinoblastoma tissues and cells. FEZF1-AS1 overexpression enhanced retinoblastoma cell viability, promoted cell cycle, and inhibited apoptosis. Conversely, FEZF1-AS1 knockdown reduced cell viability, cycle, and elevated apoptosis. The interaction between FEZF1-AS1 and microRNA-363-3p (miR-363-3p) was confirmed. FEZF1-AS1 down-regulated miR-363-3p and up-regulated PAX6. PAX6 was a target gene of miR-363-3p. EZF1-AS1 promoted retinoblastoma cell viability and suppressed apoptosis via PAX6. Further, we demonstrated that FEZF1-AS1 contribute to tumor formation in vivo. In conclusion, FEZF1-AS1 elevated growth and inhibited apoptosis by regulating miR-363-3p/PAX6 in retinoblastoma, which provide a new target for retinoblastoma treatment.

摘要

成视网膜细胞瘤是儿童眼内最常见的恶性肿瘤，发病率高。长链非编码 RNA（lncRNA）在成视网膜细胞瘤的进展中发挥重要作用。已经发现 lncRNA FEZF1 反义 RNA 1（FEZF1-AS1）可刺激成视网膜细胞瘤。然而，FEZF1-AS1 促进成视网膜细胞瘤进展的机制尚不清楚。在本研究中，FEZF1-AS1 在成视网膜细胞瘤组织和细胞中上调。FEZF1-AS1 的过表达增强了成视网膜细胞瘤细胞活力，促进了细胞周期，抑制了细胞凋亡。相反，FEZF1-AS1 的下调降低了细胞活力、周期，并提高了细胞凋亡。证实了 FEZF1-AS1 与 microRNA-363-3p（miR-363-3p）之间的相互作用。FEZF1-AS1 下调 miR-363-3p 并上调 PAX6。PAX6 是 miR-363-3p 的靶基因。EZF1-AS1 通过 PAX6 促进成视网膜细胞瘤细胞活力并抑制细胞凋亡。此外，我们还证明 FEZF1-AS1 在体内有助于肿瘤形成。总之，FEZF1-AS1 通过调节 miR-363-3p/PAX6 在成视网膜细胞瘤中促进生长并抑制细胞凋亡，为成视网膜细胞瘤的治疗提供了新的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/46dc5c0ab92d/10528_2020_10026_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/a3e65cd2fb67/10528_2020_10026_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/4730960358d1/10528_2020_10026_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/dc3da8e1436b/10528_2020_10026_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/6c7994133764/10528_2020_10026_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/5dc595881d36/10528_2020_10026_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/46dc5c0ab92d/10528_2020_10026_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/a3e65cd2fb67/10528_2020_10026_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/4730960358d1/10528_2020_10026_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/dc3da8e1436b/10528_2020_10026_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/6c7994133764/10528_2020_10026_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/5dc595881d36/10528_2020_10026_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b904/8096736/46dc5c0ab92d/10528_2020_10026_Fig6_HTML.jpg

相似文献

1
Long Non-coding RNA FEZF1-AS1 Promotes Growth and Reduces Apoptosis Through Regulation of miR-363-3p/PAX6 Axis in Retinoblastoma.长链非编码 RNA FEZF1-AS1 通过调控 miR-363-3p/PAX6 轴促进视网膜母细胞瘤的生长并减少细胞凋亡。
Biochem Genet. 2021 Jun;59(3):637-651. doi: 10.1007/s10528-020-10026-7. Epub 2021 Jan 11.
2
lncRNA ZFPM2-AS1 promotes retinoblastoma progression by targeting microRNA miR-511-3p/paired box protein 6 (PAX6) axis.长链非编码 RNA ZFPM2-AS1 通过靶向 microRNA miR-511-3p/配对盒蛋白 6 (PAX6) 轴促进视网膜母细胞瘤的进展。
Bioengineered. 2022 Jan;13(1):1637-1649. doi: 10.1080/21655979.2021.2021346.
3
LncRNA FEZF1-AS1 Modulates Cancer Stem Cell Properties of Human Gastric Cancer Through miR-363-3p/HMGA2.长链非编码RNA FEZF1-AS1通过miR-363-3p/HMGA2调节人胃癌的癌症干细胞特性。
Cell Transplant. 2020 Jan-Dec;29:963689720925059. doi: 10.1177/0963689720925059.
4
Up-Regulated LncRNA FEZF1-AS1 Promotes the Progression of Cervical Carcinoma Cells via MiR-367-3p/SLC12A5 Signal Axis.上调的长链非编码 RNA FEZF1-AS1 通过 miR-367-3p/SLC12A5 信号轴促进宫颈癌细胞的进展。
Arch Med Res. 2022 Jan;53(1):9-19. doi: 10.1016/j.arcmed.2021.05.004. Epub 2021 Aug 3.
5
LncRNA FEZF1-AS1 promotes colorectal cancer progression through regulating the miR-363-3p/PRRX1 pathway.长链非编码 RNA FEZF1-AS1 通过调控 miR-363-3p/PRRX1 通路促进结直肠癌进展。
Adv Clin Exp Med. 2021 Aug;30(8):839-848. doi: 10.17219/acem/135693.
6
The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.长链非编码 RNA FEZF1-AS1 通过调控 OTX1 并靶向 miR-30a-5p 促进结直肠癌的进展。
Oncol Res. 2020 Feb 7;28(1):51-63. doi: 10.3727/096504019X15619783964700. Epub 2019 Jul 3.
7
Long non-coding RNA FEZF1-AS1 induced progression of ovarian cancer via regulating miR-130a-5p/SOX4 axis.长链非编码 RNA FEZF1-AS1 通过调控 miR-130a-5p/SOX4 轴促进卵巢癌细胞的进展。
J Cell Mol Med. 2020 Apr;24(7):4275-4285. doi: 10.1111/jcmm.15088. Epub 2020 Mar 5.
8
MiR-365b-3p, down-regulated in retinoblastoma, regulates cell cycle progression and apoptosis of human retinoblastoma cells by targeting PAX6.miR-365b-3p 在视网膜母细胞瘤中下调，通过靶向 PAX6 调节人视网膜母细胞瘤细胞的细胞周期进程和细胞凋亡。
FEBS Lett. 2013 Jun 19;587(12):1779-86. doi: 10.1016/j.febslet.2013.04.029. Epub 2013 May 6.
9
AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1.AFAP1 反义 RNA1 通过海绵吸附 microRNA miR-545-3p 促进视网膜母细胞瘤的进展，该 microRNA 靶向 G 蛋白亚基β1。
Bioengineered. 2022 Mar;13(3):5638-5652. doi: 10.1080/21655979.2022.2033464.
10
Long non-coding RNA FEZF1-AS1 promotes cell growth in multiple myeloma via miR-610/Akt3 axis.长非编码 RNA FEZF1-AS1 通过 miR-610/Akt3 轴促进多发性骨髓瘤细胞生长。
Biomed Pharmacother. 2018 Jul;103:1727-1732. doi: 10.1016/j.biopha.2018.04.094.

引用本文的文献

1
Molecular Biological Research on the Pathogenic Mechanism of Retinoblastoma.视网膜母细胞瘤致病机制的分子生物学研究
Curr Issues Mol Biol. 2024 May 27;46(6):5307-5321. doi: 10.3390/cimb46060317.
2
Role of microRNA-363 during tumor progression and invasion.miRNA-363 在肿瘤进展和侵袭中的作用。
J Physiol Biochem. 2024 Aug;80(3):481-499. doi: 10.1007/s13105-024-01022-1. Epub 2024 May 1.
3
Role of non-coding RNAs and exosomal non-coding RNAs in retinoblastoma progression.非编码RNA和外泌体非编码RNA在视网膜母细胞瘤进展中的作用。

本文引用的文献

1
Effects of oncolytic viruses and viral vectors on immunity in glioblastoma.溶瘤病毒和病毒载体对胶质母细胞瘤免疫的影响。
Gene Ther. 2022 Apr;29(3-4):115-126. doi: 10.1038/s41434-020-00207-9. Epub 2020 Nov 15.
2
MicroRNA‑363‑3p inhibits cell proliferation and induces apoptosis in retinoblastoma cells via the Akt/mTOR signaling pathway by targeting PIK3CA.微小 RNA-363-3p 通过靶向 PIK3CA 抑制 Akt/mTOR 信号通路从而抑制视网膜母细胞瘤细胞增殖并诱导其凋亡。
Oncol Rep. 2020 May;43(5):1365-1374. doi: 10.3892/or.2020.7544. Epub 2020 Mar 12.
3
MicroRNA-129-5p suppresses proliferation, migration and invasion of retinoblastoma cells through PI3K/AKT signaling pathway by targeting PAX6.
Front Cell Dev Biol. 2022 Dec 23;10:1065837. doi: 10.3389/fcell.2022.1065837. eCollection 2022.
4
Long non-coding RNAs involved in retinoblastoma.参与视网膜母细胞瘤的长链非编码RNA
J Cancer Res Clin Oncol. 2023 Jan;149(1):401-421. doi: 10.1007/s00432-022-04398-z. Epub 2022 Oct 28.
miR-129-5p 通过靶向 PAX6 抑制视网膜母细胞瘤细胞的增殖、迁移和侵袭，该通路涉及 PI3K/AKT 信号通路。
Pathol Res Pract. 2019 Dec;215(12):152641. doi: 10.1016/j.prp.2019.152641. Epub 2019 Oct 4.
4
lncRNA Increases Proliferation and Multidrug Resistance of Retinoblastoma Cells Through Downregulating miR-513a-5p.lncRNA 通过下调 miR-513a-5p 增加视网膜母细胞瘤细胞的增殖和多药耐药性。
DNA Cell Biol. 2020 Jan;39(1):69-77. doi: 10.1089/dna.2019.5063. Epub 2019 Nov 8.
5
FEZF1-AS1 functions as an oncogenic lncRNA in retinoblastoma.FEZF1-AS1 在视网膜母细胞瘤中作为致癌 lncRNA 发挥作用。
Biosci Rep. 2019 May 31;39(5). doi: 10.1042/BSR20190754.
6
LncRNA MALAT1 promotes colorectal cancer development by sponging miR-363-3p to regulate EZH2 expression.长链非编码 RNA MALAT1 通过海绵吸附 miR-363-3p 来调节 EZH2 表达，促进结直肠癌的发展。
J Biol Regul Homeost Agents. 2019 Mar-Apr;33(2):331-343.
7
Therapeutic targeting of the RB1 pathway in retinoblastoma with the oncolytic adenovirus VCN-01.溶瘤腺病毒 VCN-01 对视网膜母细胞瘤中 RB1 通路的治疗靶向作用。
Sci Transl Med. 2019 Jan 23;11(476). doi: 10.1126/scitranslmed.aat9321.
8
Highly expressed lncRNA FAL1 promotes the progression of gastric cancer by inhibiting PTEN.高表达的长链非编码 RNA FAL1 通过抑制 PTEN 促进胃癌的进展。
Eur Rev Med Pharmacol Sci. 2018 Dec;22(23):8257-8264. doi: 10.26355/eurrev_201812_16520.
9
Curcumin inhibits proliferation, migration, invasion and promotes apoptosis of retinoblastoma cell lines through modulation of miR-99a and JAK/STAT pathway.姜黄素通过调节 miR-99a 和 JAK/STAT 通路抑制视网膜母细胞瘤细胞系的增殖、迁移、侵袭并促进其凋亡。
BMC Cancer. 2018 Dec 10;18(1):1230. doi: 10.1186/s12885-018-5130-y.
10
α-pinene regulates and induces G/M phase cell cycle arrest in human hepatocellular carcinoma cells.α-蒎烯调控并诱导人肝癌细胞 G/M 期细胞周期阻滞。
Biosci Rep. 2018 Dec 11;38(6). doi: 10.1042/BSR20180980. Print 2018 Dec 21.