分析非小细胞肺癌患者外周血中 miR-19 和 miR-21 的表达水平及其诊断价值。

Analysis on expression level and diagnostic value of miR-19 and miR-21 in peripheral blood of patients with undifferentiated lung cancer.

机构信息

Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China.

出版信息

Eur Rev Med Pharmacol Sci. 2018 Jan;22(23):8367-8373. doi: 10.26355/eurrev_201801_16534.

DOI:10.26355/eurrev_201801_16534

PMID:30556877

Abstract

OBJECTIVE

This paper aims to investigate the expression level and diagnostic value of miR-19 miR-21 in peripheral blood of patients with undifferentiated lung cancer.

PATIENTS AND METHODS

58 patients with undifferentiated lung cancer hospitalized in the oncology department of The First Affiliated Hospital of Nanchang University from September 2014 to May 2017 were selected as the experimental group, and 42 healthy volunteers in the same period as the control group at the same time. General clinical data in the two groups were collected. The expression levels of miR-19 and miR-21 in peripheral blood of the two groups were measured by Real-Time fluorescence quantitative Polymerase Chain Reaction. The expression levels of peripheral blood miR-19 and miR-21 in large and small cell lung cancer of undifferentiated lung cancer were analyzed and compared. ROC curve was used to analyze the diagnostic value of miR-19 and miR-21 in undifferentiated lung cancer.

RESULTS

The expression levels of miR-19 and miR-21 in peripheral blood of the experimental group were significantly higher than those of the control group (p<0.05). The AUC of miR-19 in the diagnosis of undifferentiated lung cancer was 0.854; the sensitivity was 98.30%; the specificity was 54.29% and the cut off was 3.54. The AUC of miR-21 in the diagnosis of undifferentiated lung cancer was 0.923; the sensitivity was 86.20%; the specificity was 76.19% and the cut off was 3.89. The AUC of combined detection in the diagnosis of undifferentiated lung cancer was 0.952; the sensitivity was 86.60%; the specificity was 97.62% and the cut off was 0.68. The specificity of combined detection was higher than that of miR-19 and miR-21 (p<0.05).

CONCLUSIONS

MiR-19 and miR-21 are highly expressed in peripheral blood of patients with undifferentiated lung cancer; miR-19 and miR-21 are expected to be used as diagnostic markers for undifferentiated lung cancer.

摘要

目的

本研究旨在探讨 miR-19 和 miR-21 在未分化型肺癌患者外周血中的表达水平及其诊断价值。

方法

选取 2014 年 9 月至 2017 年 5 月南昌大学第一附属医院肿瘤科收治的 58 例未分化型肺癌患者作为实验组，同时选取同期 42 例健康志愿者作为对照组。收集两组患者的一般临床资料。采用实时荧光定量聚合酶链反应检测两组患者外周血中 miR-19 和 miR-21 的表达水平。分析并比较未分化型肺癌中大细胞肺癌和小细胞肺癌患者外周血 miR-19 和 miR-21 的表达水平。采用 ROC 曲线分析 miR-19 和 miR-21 对未分化型肺癌的诊断价值。

结果

实验组患者外周血 miR-19 和 miR-21 的表达水平明显高于对照组（p<0.05）。miR-19 诊断未分化型肺癌的 AUC 为 0.854，灵敏度为 98.30%，特异度为 54.29%，截断值为 3.54。miR-21 诊断未分化型肺癌的 AUC 为 0.923，灵敏度为 86.20%，特异度为 76.19%，截断值为 3.89。联合检测诊断未分化型肺癌的 AUC 为 0.952，灵敏度为 86.60%，特异度为 97.62%，截断值为 0.68。联合检测的特异度高于 miR-19 和 miR-21（p<0.05）。