Zhou Yingyan, Shen Shimo
Department of Respiration, Ningbo Medical Center Lihuili Hospital, Ningbo City, Zhejiang, China.
Medicine (Baltimore). 2019 Jul;98(30):e16546. doi: 10.1097/MD.0000000000016546.
Lung cancer is a malignant tumor with high morbidity and mortality. Early diagnosis remains a great challenge for the cancer. In this study, we aimed to explore diagnostic performance of serum microRNA-520f (miR-520f) in lung cancer.Serum specimens were collected from 139 lung cancer patients and 76 healthy volunteers. Relative expression level of serum miR-520f was detected adopting quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was applied to evaluate the association of miR-520f with clinical parameters of the patients. Additionally, receiver operating characteristic (ROC) analysis was performed to evaluate diagnostic value of miR-520f in lung cancer.Serum miR-520f was down-regulated in lung cancer patients compared with healthy group (P <.001). Moreover, the expression of miR-520f was significantly associated with advanced TNM stage (P = .031) and metastasis (P = .002). The area under the curve (AUC) value of ROC curve was 0.888, suggesting that miR-520f could be a diagnostic biomarker for lung cancer. The cut-off value of serum miR-520f for lung cancer diagnosis was 1.815, with a sensitivity of 79.9% and a specificity of 84.2%.Serum miR-520f was down-regulated in lung cancer patients, and may be a candidate biomarker for non-invasive screening of the disease.
肺癌是一种发病率和死亡率都很高的恶性肿瘤。早期诊断仍然是该癌症面临的巨大挑战。在本研究中，我们旨在探索血清微小RNA-520f（miR-520f）在肺癌中的诊断性能。收集了139例肺癌患者和76名健康志愿者的血清标本。采用定量实时聚合酶链反应（qRT-PCR）检测血清miR-520f的相对表达水平。应用卡方检验评估miR-520f与患者临床参数的相关性。此外，进行了受试者工作特征（ROC）分析以评估miR-520f在肺癌中的诊断价值。与健康组相比，肺癌患者血清miR-520f表达下调（P<0.001）。此外，miR-520f的表达与晚期TNM分期（P=0.031）和转移（P=0.002）显著相关。ROC曲线的曲线下面积（AUC）值为0.888，表明miR-520f可能是肺癌的诊断生物标志物。血清miR-520f用于肺癌诊断的截断值为1.815，灵敏度为79.9%，特异性为84.2%。肺癌患者血清miR-520f表达下调，可能是该疾病非侵入性筛查的候选生物标志物。
