Second Liver Cirrhosis Diagnosis and Treatment Center, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
Medical Laboratory of Shenzhen LuoHu People's Hospital, Shenzhen, China.
J Cell Physiol. 2019 Aug;234(8):12964-12970. doi: 10.1002/jcp.27963. Epub 2018 Dec 17.
Hepatocellular carcinoma is one of the most prevalent and fatal cancers. Studying the long noncoding RNA (lncRNA) alterations in hepatocellular carcinoma may lead to new therapeutic strategies. We checked whether there were correlations between The Cancer Genome Atlas expression profiles of the differentially expressed lncRNAs and their DNA methylation status or the copy number variations for hepatocellular carcinoma. We obtained 41 lncRNAs that were differentially expressed between tumor and normal samples, and their DNA methylation status was negatively correlated with the expression levels. We identified five lncRNAs that were recurrently amplified or deleted in tumor samples, but none of them were associated with the messenger RNA (mRNA) expression levels. To obtain the biological function of these lncRNAs, the coexpressed mRNAs in the hepatocellular carcinoma were figured out. A total of 10 lncRNAs were highly correlated with at least one gene. Six out of the ten lncRNAs were already known to be related with cancer previously. LINC01615 had 72 coexpressed genes, and we carried out the gene ontology (GO) term enrichment for these protein-coding genes. The results suggested that these lncRNAs were associated with extracellular matrix organization. To summarize, we identified 41 potentially cancer-related lncRNAs. In particular, we proposed that LINC01615 potentially affected the extracellular matrix and had further impacts on the metastasis of hepatocellular carcinoma.
肝细胞癌是最常见和致命的癌症之一。研究肝细胞癌中长非编码 RNA（lncRNA）的改变可能会导致新的治疗策略。我们检查了差异表达的 lncRNA 的 The Cancer Genome Atlas 表达谱与它们在肝细胞癌中的 DNA 甲基化状态或拷贝数变异之间是否存在相关性。我们获得了 41 个在肿瘤和正常样本之间表达差异的 lncRNA，它们的 DNA 甲基化状态与表达水平呈负相关。我们鉴定了五个在肿瘤样本中反复扩增或缺失的 lncRNA，但它们都与信使 RNA（mRNA）表达水平无关。为了获得这些 lncRNA 的生物学功能，我们分析了肝细胞癌中的共表达 mRNA。共有 10 个 lncRNA 与至少一个基因高度相关。这 10 个 lncRNA 中有 6 个以前已经与癌症有关。LINC01615 有 72 个共表达基因，我们对这些编码蛋白的基因进行了基因本体（GO）术语富集。结果表明，这些 lncRNA 与细胞外基质组织有关。总之，我们鉴定了 41 个可能与癌症相关的 lncRNA。特别是，我们提出 LINC01615 可能影响细胞外基质，并对肝细胞癌的转移产生进一步影响。
