OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China.
OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China.
Virus Res. 2019 Feb;261:50-55. doi: 10.1016/j.virusres.2018.12.008. Epub 2018 Dec 14.
Peste des petits ruminants and cystic hydatidosis may be simultaneously endemic in a given area. Their pathogens are small ruminant morbillivirus (SRMV) and Echinococcus granulosus (E. granulosus), respectively. The SRMV, formerly called peste-des-petits-ruminants virus (PPRV), is classified into the genus Morbillivirus in the family Paramyxoviridae. This virus is an ideal vaccine vector to deliver immunogenic proteins. In this study, a reverse genetics system was developed to rescue a recombinant SRMV (Nigeria 75/1 strain) expressing E. granulosus EG95 antigen in vitro. The recombinant SRMV, albeit replicating more slowly than its parental virus, could effectively express the EG95 antigen in cells by analyses of Western blot, indirect immunofluorescence and mass spectrometry. An EG95 subunit vaccine has been widely used for prevention of cystic hydatidosis in some areas of China. The EG95-expressing SRMV, if proven to induce effective immune responses against both diseases in a future animal experiment, would become a potential candidate of bivalent vaccine.
绵羊肺腺瘤病和泡状棘球蚴病可能在同一地区同时流行。它们的病原体分别是小反刍兽瘟病毒（small ruminant morbillivirus，SRMV）和细粒棘球绦虫（Echinococcus granulosus，E. granulosus）。该 SRMV 以前被称为绵羊肺腺瘤病病毒（peste-des-petits-ruminants virus，PPRV），属于副黏病毒科麻疹病毒属。该病毒是一种理想的疫苗载体，可传递免疫原性蛋白。在这项研究中，开发了一种反向遗传学系统，以体外拯救表达细粒棘球绦虫 EG95 抗原的重组小反刍兽瘟病毒（尼日利亚 75/1 株）。尽管重组 SRMV 的复制速度比其亲本病毒慢，但通过 Western blot、间接免疫荧光和质谱分析，它可以有效地在细胞中表达 EG95 抗原。EG95 亚单位疫苗已在中国一些地区广泛用于预防泡状棘球蚴病。如果在未来的动物实验中证明表达 EG95 的 SRMV 能有效诱导对这两种疾病的免疫反应，它将成为双价疫苗的潜在候选疫苗。
