Virus Research Unit, Department of Microbiology and Immunology, The University of Otago, PO Box 56, Dunedin, New Zealand.
Vaccine. 2012 Jan 5;30(2):398-406. doi: 10.1016/j.vaccine.2011.10.079. Epub 2011 Nov 12.
The parapoxvirus, orf virus (ORFV) causes superficial skin lesions in infected sheep. Unattenuated ORFV is used globally to vaccinate against orf. Recombinant poxviruses are proven delivery systems and we investigated strategies to express the immunogenic Echinococcus granulosus peptide EG95 from ORFV with the aim of developing a recombinant bivalent vaccine. EG95 is an oncosphere protein of the cestode E. granulosus, a parasite responsible for causing cystic hydatid disease in a wide range of hosts including humans and grazing animals such as sheep. Recombinant viruses were produced in which EG95 was expressed by itself or fused to ORFV envelope-associated structural proteins 10 kDa and F1L. Infection studies in sheep showed that specific antibodies were produced against ORFV and EG95 and that the antibody levels against EG95 were comparable to that of animals immunized with purified EG95 in Quil A adjuvant, an immunization regime that is known to afford protection. A single exposure to the dual vaccine has potential for protecting lambs against orf and for priming against EG95 so as to respond strongly to a later injection of EG95 protein.
副痘病毒（ORFV）可导致感染绵羊的皮肤浅层病变。未减毒的 ORFV 在全球范围内被用于接种以预防羊痘。重组痘病毒是已证实的有效疫苗传递系统，我们研究了用其表达免疫原性棘球蚴多肽 EG95 的策略，旨在开发一种重组双价疫苗。EG95 是绦虫棘球蚴的原头节蛋白，该寄生虫可导致广泛宿主（包括人类和放牧动物如绵羊）发生囊性包虫病。我们生产了表达 EG95 自身或与 ORFV 包膜相关结构蛋白 10 kDa 和 F1L 融合的重组病毒。绵羊感染研究表明，针对 ORFV 和 EG95 产生了特异性抗体，针对 EG95 的抗体水平与用 Quil A 佐剂免疫的动物相当，该免疫方案已知可提供保护。单次接触双价疫苗有潜力预防羔羊的羊痘感染，并针对 EG95 进行初次免疫，以便对随后的 EG95 蛋白注射产生强烈反应。
