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木脂素通过 NF-κB、AP-1 和 IRF3 抑制脂多糖刺激的 RAW264.7 细胞中炎症介质的产生。

Lignans Suppresses the Production of Inflammatory Mediators Regulated by NF-κB, AP-1, and IRF3 in Lipopolysaccharide-Stimulated RAW264.7 Cells.

机构信息

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.

College of Professional and Continuing Education, Hong Kong Polytechnic University, Hong Kong 999077, China.

出版信息

Molecules. 2018 Dec 14;23(12):3319. doi: 10.3390/molecules23123319.

DOI:10.3390/molecules23123319
PMID:30558163
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6320760/
Abstract

Schisandra Fructus (SF) is a traditional Chinese herb used in the treatment of inflammatory disorders like hepatitis. One of the main anti-inflammatory components of SF is the lignans. However, the underlying anti-inflammatory mechanism of lignans (SCL) remains unclear. This study aims to investigate the effects of SCL on inflammatory mediators in lipopolysaccharide-stimulated RAW264.7 cells and explore the underlying mechanism. The production of nitric oxide (NO) was determined by Griess reaction. ELISA was used to determine cytokine levels and chemokines secretion. To estimate protein levels and enzyme activities, we employed Western blotting. Nuclear localization of NF-κB, AP-1, and IRF3 was detected using immunofluorescence analyses. The results showed that SCL significantly reduced the release of inflammatory mediators, including NO and PGE2, which may be related to down-regulation of iNOS and COX-2 expression. The production of cytokines and chemokines was suppressed by SCL treatment. SCL also decreased the phosphorylation of IKKα/β, IκB-α, Akt, TBK1, ERK, p38, JNK, NF-κB (p65), AP-1 (c-Jun), and IRF3 in RAW264.7 macrophages activated with LPS. The nuclear protein levels and nuclear translocation of AP-1, NF-κB and IRF3 were suppressed by SCL. These results indicated that SCL suppressed the IKKα/β/NF-κB, MAPKs/AP-1 and TBK1/IRF3 signaling pathways in LPS-stimulated RAW264.7 macrophages.

摘要

五味子（SF）是一种传统的中药，用于治疗肝炎等炎症性疾病。SF 的主要抗炎成分之一是木脂素。然而，木脂素（SCL）的抗炎机制尚不清楚。本研究旨在探讨 SCL 对脂多糖刺激的 RAW264.7 细胞中炎症介质的影响，并探讨其潜在机制。通过格里塞反应测定一氧化氮（NO）的产生。酶联免疫吸附试验（ELISA）用于测定细胞因子水平和趋化因子分泌。采用 Western blot 估计蛋白质水平和酶活性。用免疫荧光分析检测 NF-κB、AP-1 和 IRF3 的核定位。结果表明，SCL 显著降低了炎症介质的释放，包括 NO 和 PGE2，这可能与 iNOS 和 COX-2 表达下调有关。SCL 处理抑制了细胞因子和趋化因子的产生。SCL 还降低了 LPS 激活的 RAW264.7 巨噬细胞中 IKKα/β、IκB-α、Akt、TBK1、ERK、p38、JNK、NF-κB（p65）、AP-1（c-Jun）和 IRF3 的磷酸化。SCL 抑制了 RAW264.7 巨噬细胞中 LPS 刺激的 IKKα/β/NF-κB、MAPKs/AP-1 和 TBK1/IRF3 信号通路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/d9656b801e07/molecules-23-03319-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/f118a5c2cc74/molecules-23-03319-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/4be682772272/molecules-23-03319-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/ea0d40569f37/molecules-23-03319-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/c99154b8b6d6/molecules-23-03319-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/e70417bfe5bf/molecules-23-03319-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/7d5055126e41/molecules-23-03319-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/27efc20b9eda/molecules-23-03319-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/5b4a2d13b220/molecules-23-03319-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/d9656b801e07/molecules-23-03319-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/f118a5c2cc74/molecules-23-03319-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/4be682772272/molecules-23-03319-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/ea0d40569f37/molecules-23-03319-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/c99154b8b6d6/molecules-23-03319-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/e70417bfe5bf/molecules-23-03319-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/7d5055126e41/molecules-23-03319-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/27efc20b9eda/molecules-23-03319-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/5b4a2d13b220/molecules-23-03319-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a170/6320760/d9656b801e07/molecules-23-03319-g009.jpg

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2
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PLoS One. 2018 Jun 11;13(6):e0198943. doi: 10.1371/journal.pone.0198943. eCollection 2018.
3
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4
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6
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8
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9
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10
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Med Res Rev. 2021 Jan;41(1):630-703. doi: 10.1002/med.21743. Epub 2020 Oct 25.
NF-κB signaling in inflammation.
NF-κB 信号转导与炎症
Signal Transduct Target Ther. 2017;2:17023-. doi: 10.1038/sigtrans.2017.23. Epub 2017 Jul 14.
4
Complementary and Alternative Therapies for Inflammatory Diseases.炎症性疾病的补充和替代疗法
Evid Based Complement Alternat Med. 2016;2016:8324815. doi: 10.1155/2016/8324815. Epub 2016 Dec 25.
5
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PLoS One. 2016 May 19;11(5):e0155879. doi: 10.1371/journal.pone.0155879. eCollection 2016.
6
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Int Immunopharmacol. 2016 Jun;35:210-216. doi: 10.1016/j.intimp.2016.04.003. Epub 2016 Apr 16.
7
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8
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J Ethnopharmacol. 2015 Nov 4;174:195-9. doi: 10.1016/j.jep.2015.08.018. Epub 2015 Aug 20.
9
NF-κB and IRF pathways: cross-regulation on target genes promoter level.核因子-κB与干扰素调节因子信号通路：靶基因启动子水平的交叉调控
BMC Genomics. 2015 Apr 17;16(1):307. doi: 10.1186/s12864-015-1511-7.
10
Molecular mechanisms that influence the macrophage m1-m2 polarization balance.影响巨噬细胞M1-M2极化平衡的分子机制。
Front Immunol. 2014 Nov 28;5:614. doi: 10.3389/fimmu.2014.00614. eCollection 2014.