Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2018 Dec 14;19(12):4059. doi: 10.3390/ijms19124059.
The plant-specific WRKY transcriptional regulatory factors have been proven to play vital roles in plant growth, development, and responses to biotic and abiotic stresses. However, there are few studies on the gene family in sugarcane ( spp.). In the present study, the characterization of a new subgroup, IIc WRKY protein ScWRKY3, from a hybrid cultivar is reported. The ScWRKY3 protein was localized in the nucleus of leaves and showed no transcriptional activation activity and no toxic effects on the yeast strain Y2HGold. An interaction between ScWRKY3 and a reported sugarcane protein ScWRKY4, was confirmed in the nucleus. The gene had the highest expression level in sugarcane stem pith. The transcript of was stable in the smut-resistant hybrid cultivar Yacheng05-179, while it was down-regulated in the smut-susceptible hybrid cultivar ROC22 during inoculation with the smut pathogen () at 0⁻72 h. was remarkably up-regulated by sodium chloride (NaCl), polyethylene glycol (PEG), and plant hormone abscisic acid (ABA), but it was down-regulated by salicylic acid (SA) and methyl jasmonate (MeJA). Moreover, transient overexpression of the gene in indicated a negative regulation during challenges with the fungal pathogen var. or the bacterial pathogen in . The findings of the present study should accelerate future research on the identification and functional characterization of the WRKY family in sugarcane.
植物特有的 WRKY 转录调控因子已被证明在植物的生长、发育以及对生物和非生物胁迫的响应中起着至关重要的作用。然而，关于甘蔗（ spp.）基因家族的研究较少。本研究报告了从杂种品种中鉴定出的一个新亚群 IIc WRKY 蛋白 ScWRKY3。ScWRKY3 蛋白定位于叶片的细胞核中，没有转录激活活性，也没有对酵母菌株 Y2HGold 产生毒性作用。在细胞核中证实了 ScWRKY3 与已报道的甘蔗蛋白 ScWRKY4 之间的相互作用。该基因在甘蔗茎髓中的表达水平最高。在抗黑粉病的杂种品种 Yacheng05-179 中， 的转录本稳定，而在易感黑粉病的杂种品种 ROC22 中，在接种黑粉病菌（）的 0⁻72 小时内，其表达下调。NaCl、PEG 和植物激素脱落酸（ABA）显著上调 ，而水杨酸（SA）和茉莉酸甲酯（MeJA）下调。此外，在 中瞬时过表达 基因表明其在真菌病原体 var. 或细菌病原体 的挑战下呈负调控。本研究的发现应加速对甘蔗 WRKY 家族的鉴定和功能特征的研究。
