Zheng Lilei, Qiu Bingling, Su Linlin, Wang Hanlin, Cui Xiuming, Ge Feng, Liu Diqiu
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Yunnan Provincial Key Laboratory of Panax Notoginseng, Kunming, China.
Front Plant Sci. 2022 Jul 14;13:930644. doi: 10.3389/fpls.2022.930644. eCollection 2022.
(Burk) F.H. Chen is a rare and valuable Chinese herb, but root rot mainly caused by severely affects the yield and quality of herbal materials. In this study, we isolated 30 WRKY transcription factors (TFs), which were divided into three groups (I, II, and III) on the basis of a phylogenetic analysis. The expression levels of 10 genes, including , in roots increased in response to a methyl jasmonate (MeJA) treatment and the following infection. Additionally, PnWRKY9 was functionally characterized. The PnWRKY9 protein was localized to the nucleus. The overexpression of in tobacco () considerably increased the resistance to , whereas an RNAi-mediated decrease in the expression level in leaves increased the susceptibility to . The RNA sequencing and hormone content analyses of -overexpression tobacco revealed that PnWRKY9 and the jasmonic acid (JA) signaling pathway synergistically enhance disease resistance. The PnWRKY9 recombinant protein was observed to bind specifically to the W-box sequence in the promoter of a JA-responsive and resistance-related defensin gene (). A yeast one-hybrid assay indicated that PnWRKY9 can activate the transcription of . Furthermore, a co-expression assay in tobacco using β-glucuronidase (GUS) as a reporter further verified that PnWRKY9 positively regulates expression. Overall, in this study, we identified WRKY TFs and demonstrated that PnWRKY9 positively affects plant defenses against the root rot pathogen. The data presented herein provide researchers with fundamental information regarding the regulatory mechanism mediating the coordinated activities of WRKY TFs and the JA signaling pathway in responses to the root rot pathogen.
（伯克）茯苓是一种珍稀名贵的中药材，但主要由[病原体名称未给出]引起的根腐病严重影响了药材的产量和质量。在本研究中，我们分离出30个WRKY转录因子（TFs），基于系统发育分析将其分为三组（I、II和III）。包括[基因名称未给出]在内的10个基因在根中的表达水平在茉莉酸甲酯（MeJA）处理及随后的[病原体名称未给出]感染后升高。此外，对PnWRKY9进行了功能表征。PnWRKY9蛋白定位于细胞核。在烟草（[烟草品种未给出]）中过表达[基因名称未给出]显著增强了对[病原体名称未给出]的抗性，而RNA干扰介导的[基因名称未给出]在叶片中的表达水平降低则增加了对[病原体名称未给出]的易感性。对过表达[基因名称未给出]的烟草进行RNA测序和激素含量分析表明，PnWRKY9与茉莉酸（JA）信号通路协同增强抗病性观察到PnWRKY9重组蛋白能特异性结合茉莉酸应答和抗病相关防御素基因（[基因名称未给出]）启动子中的W盒序列。酵母单杂交试验表明，PnWRKY9可激活[基因名称未给出]的转录。此外，以β - 葡萄糖醛酸酶（GUS）为报告基因在烟草中进行的共表达试验进一步证实，PnWRKY9正向调控[基因名称未给出]的表达。总体而言，在本研究中，我们鉴定了[转录因子数量未明确]个WRKY TFs，并证明PnWRKY9对植物抵御根腐病菌具有积极作用。本文提供的数据为研究人员提供了关于WRKY TFs与JA信号通路在植物对根腐病菌应答中协同作用的调控机制的基础信息。
