School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
College of Nursing, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Molecules. 2018 Dec 15;23(12):3333. doi: 10.3390/molecules23123333.
With the diversity of modern dietary lifestyles, digestive system disorders (DSD) have become a frequently occurring disease in recent years. Astragalus polysaccharide (APS) is a homogeneous polysaccharide extracted from , which might ameliorate the digestive and absorptive functions. However, the treatment mechanisms remain unclear. In this study, rats with DSD were fed a high-fat⁻low-protein diet and subjected to weight-bearing swimming until exhaustion. When body weight and autonomous activities of the rats decreased, they were administered APS. After two weeks, serum metabolomics analysis based on LC-MS was performed to validate the therapeutic effect of APS and explore its mechanism. APS pharmacodynamics was determined in this study, and serum metabolomics analysis discovered and identified 16 significant, differentially produced metabolites involved in energy, amino acid, and lipid metabolism, including citric acid, lactic acid, alanine, phosphatidylcholine, phenylalanine. After treatment with APS, the levels of the above small-molecule metabolites were reversed. Our results show the efficacy of APS in DSD treatment through the regulation of perturbed metabolic pathways related to energy, amino acid, and lipid metabolism.
随着现代饮食生活方式的多样化，近年来消化系统疾病（DSD）已成为一种常见病。黄芪多糖（APS）是从 中提取的均一多糖，可能改善消化和吸收功能。然而，其治疗机制尚不清楚。在这项研究中，DSD 大鼠喂食高脂肪-低蛋白饮食，并进行负重游泳直至力竭。当大鼠体重和自主活动减少时，给予 APS。两周后，进行基于 LC-MS 的血清代谢组学分析，以验证 APS 的治疗效果并探讨其机制。本研究测定了 APS 的药效学，血清代谢组学分析发现并鉴定了 16 种与能量、氨基酸和脂质代谢相关的显著差异产生的代谢物，包括柠檬酸、乳酸、丙氨酸、磷脂酰胆碱、苯丙氨酸。APS 治疗后，上述小分子代谢物的水平得到逆转。我们的结果表明，APS 通过调节与能量、氨基酸和脂质代谢相关的扰动代谢途径，对 DSD 治疗具有疗效。
