Sant Longowal Institute of Engineering and Technology, Longowal, Punjab 148106, India.
Sant Longowal Institute of Engineering and Technology, Longowal, Punjab 148106, India.
Ultrason Sonochem. 2021 Jun;74:105572. doi: 10.1016/j.ultsonch.2021.105572. Epub 2021 Apr 22.
From the recent market trend, there is a huge demand for the bioactive compounds from various food matrices that could be capable enough to combat the emerging health effects in day-to-day life. Fenugreek is a well-known spice from ancient times for its medicinal and health benefits. In the present study, two methods of green extraction microwave (MAE) and ultrasound (UAE) assisted were studied in regard of extraction of fenugreek diosgenin. In this study, solvent type (acetone, ethanol, hexane and petroleum ether), solvent concentration (40, 60, 80 and 100%) and treatment time (1.5, 3.0, 4.5 and 6.0 min and 30, 40, 50 and 60 min for MAE and UAE method respectively) was varied to observe the effect of these parameters over extract yield and diosgenin content. The results of this study revealed that treatment time, type of solvent and its concentration and method adopted for extraction of diosgenin has significant effect. In relation with better yield extract and diosgenin content, the yield of fenugreek seed extract was 7.83% with MAE and 21.48% with UAE of fenugreek seed powder at 80% ethanol concentration at 6 and 60 min respectively. The content of diosgenin was observed in fenugreek seed powder extract was 35.50 mg/100 g in MAE and 40.37 mg/100 g in UAE with 80% ethanol concentration at 6 and 60 min respectively. The overall range of yield of fenugreek extract was varied from 1.04% to 32.48% and diosgenin content was 15.82 mg/100 g to 40.37 mg/100 g of fenugreek seed powder including both extraction methods. This study revealed that UAE would impose better ways for preparing fenugreek extract and observing diosgenin content from fenugreek seeds.
从最近的市场趋势来看，人们对各种食物基质中的生物活性化合物有巨大的需求，这些化合物有足够的能力应对日常生活中的新出现的健康影响。葫芦巴是一种从古至今因其药用和健康益处而闻名的香料。在本研究中，研究了两种绿色提取方法——微波（MAE）和超声（UAE）辅助提取葫芦巴薯蓣皂苷元。在这项研究中，改变了溶剂类型（丙酮、乙醇、正己烷和石油醚）、溶剂浓度（40%、60%、80%和 100%）和处理时间（1.5、3.0、4.5 和 6.0 分钟和 MAE 和 UAE 方法的 30、40、50 和 60 分钟），以观察这些参数对提取产率和薯蓣皂苷元含量的影响。研究结果表明，处理时间、溶剂类型和浓度以及提取薯蓣皂苷元的方法对提取产率和薯蓣皂苷元含量有显著影响。在与更好的产率和薯蓣皂苷元含量相关的方面，在 80%乙醇浓度下，MAE 法提取葫芦巴种子的产率为 7.83%，UAE 法为 21.48%，时间分别为 6 分钟和 60 分钟。在 MAE 中，在 80%乙醇浓度下，6 分钟和 60 分钟时，葫芦巴种子粉提取物中薯蓣皂苷元的含量分别为 35.50mg/100g 和 40.37mg/100g。在 UAE 中，在 80%乙醇浓度下，6 分钟和 60 分钟时，葫芦巴种子粉提取物中薯蓣皂苷元的含量分别为 35.50mg/100g 和 40.37mg/100g。两种方法的总产率范围为 1.04%至 32.48%，薯蓣皂苷元含量为 15.82mg/100g 至 40.37mg/100g 的葫芦巴种子粉。本研究表明，UAE 更适合用于制备葫芦巴提取物并观察葫芦巴种子中的薯蓣皂苷元含量。
