Miyamoto K, French S W
Department of Pathology, School of Medicine, Faculty of Health Sciences, University of Ottawa, Canada.
Alcohol Clin Exp Res. 1988 Aug;12(4):512-5. doi: 10.1111/j.1530-0277.1988.tb00234.x.
The level of adenosine was measured in monthly biopsied livers from rats fed ethanol and a high fat/low protein diet in order to test a hypothesis that hepatic adenosine is increased due to enhanced breakdown of adenine nucleotides in which ATP and total adenylate pool were decreased by chronic ethanol feeding. The ethanol-fed rats showed a significantly higher average level of adenosine compared to the pair-fed controls. When investigated monthly, however, adenosine in ethanol-fed rats increased only after the decrease in ATP had stabilized and AMP remained unchanged, indicating that these changes were not temporarily related. The average percentage of change in adenosine after acute hyperoxia or hypoxia were variable both in ethanol-fed and pair-fed rats. There was a tendency for a positive correlation between the percentage of change of adenosine and AMP after hyperoxia regardless of ethanol feeding. A negative correlation between the percentage of change of adenosine and energy charge, and a positive correlation between the percentage of change of adenosine and AMP were seen after hypoxia regardless of ethanol feeding. Adenosine levels changed rapidly in response to changes in systemic of pO2 in both the ethanol-fed and control rats, indicating that the liver maintained its normal response to the changes in energy state. The results indicate that chronic ethanol feeding does increase the level of adenosine in the liver and that this level remains responsive to acute changes in pO2.(ABSTRACT TRUNCATED AT 250 WORDS)
为了验证一个假说，即慢性乙醇喂养导致三磷酸腺苷（ATP）和总腺苷酸池减少，进而通过增强腺嘌呤核苷酸的分解使肝脏腺苷增加，研究人员对喂食乙醇和高脂/低蛋白饮食的大鼠每月进行肝脏活检，并测定腺苷水平。与配对喂养的对照组相比，喂食乙醇的大鼠腺苷平均水平显著更高。然而，每月进行研究时，喂食乙醇的大鼠体内腺苷仅在ATP水平下降稳定且一磷酸腺苷（AMP）保持不变后才增加，这表明这些变化并非暂时相关。急性高氧或低氧后，喂食乙醇和配对喂养的大鼠腺苷变化的平均百分比均有所不同。无论是否喂食乙醇，高氧后腺苷变化百分比与AMP之间均有正相关趋势。无论是否喂食乙醇，低氧后腺苷变化百分比与能荷之间呈负相关，腺苷变化百分比与AMP之间呈正相关。喂食乙醇的大鼠和对照大鼠的腺苷水平均随全身氧分压（pO2）变化而迅速改变，这表明肝脏对能量状态变化保持正常反应。结果表明，慢性乙醇喂养确实会增加肝脏中腺苷的水平，且该水平对pO2的急性变化仍有反应。（摘要截选至250字）
