Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu 610041 , China.
University of Chinese Academy of Sciences , Beijing 100049 , China.
J Agric Food Chem. 2019 Jan 16;67(2):563-577. doi: 10.1021/acs.jafc.8b05104. Epub 2019 Jan 2.
Plants have inherent tolerance to UV stress. However, very limited information is available about how wheat perceives and defends against UV disaster. To obtain the molecular mechanisms underlying UV tolerance of wheat, the phenotypic and transcriptomic responses of ZN168 and ZKM138 with contrasting UV tolerance were characterized. Compared with ZKM138, ZN168 showed significantly less UV damage. High-throughput sequencing revealed that UV stress inhibited the expression of genes related to photosynthesis and carbon fixation and a less degree for ZN168 than ZKM138. The distinctive performance of ZN168 is mediated by the selective expression of genes involved in multiple defense responses. Besides, metabolome analysis on grains suggested that UV radiation had a significant effect on anthocyanin accumulation. This study will enable us to exploit genes pinpointed as the targets of genetic engineering, thereby improving the UV tolerance of wheat. Furthermore, the anthocyanin-enriched wheat can be excellent resources to act as functional food.
植物对 UV 胁迫具有内在的耐受性。然而，关于小麦如何感知和抵御 UV 灾害的信息非常有限。为了获得小麦耐 UV 的分子机制，对具有不同耐 UV 性的 ZN168 和 ZKM138 的表型和转录组响应进行了表征。与 ZKM138 相比，ZN168 表现出的 UV 损伤明显较少。高通量测序表明，UV 胁迫抑制了与光合作用和碳固定相关的基因的表达，ZN168 的程度比 ZKM138 低。ZN168 的独特表现是通过选择性表达参与多种防御反应的基因来介导的。此外，对籽粒的代谢组分析表明，UV 辐射对花色苷积累有显著影响。本研究将使我们能够利用被确定为遗传工程目标的基因，从而提高小麦的耐 UV 性。此外，富含花色苷的小麦可以成为极好的功能性食品资源。
