Department of Laboratory Medicine, West China Second University Hospital, Chengdu, Sichuan, China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, China.
J Transl Med. 2018 Dec 18;16(1):362. doi: 10.1186/s12967-018-1716-z.
Infection with the hepatitis B virus (HBV) is an independent risk factor for liver cirrhosis and hepatocellular carcinoma, polymorphisms in HLA-DQB1 play an important role in HBV infections.
This study examined the relationships between HLA-DQB1 alleles and HBV infection susceptibility among 256 HBV carriers and 433 healthy controls. Venous blood samples were subjected to DQB1 high-resolution typing and testing for interferon-gamma, interleukin-4 (IL-4), interleukin-10, and DQB1 mRNA expression. A meta-analysis was also performed using relevant case-control studies that evaluated the associations of HLA-DQB1 alleles with HBV infection and clearance.
We found that HLA-DQB106:03 protected against HBV infection. Levels of IFN-γ and IL-4 were significantly elevated in HBV cases with HLA-DQB106:05 (vs. HLA-DQB105:03), and the HBV group had higher DQB1 mRNA expression than the healthy control group with HLA-DQB105:03 and HLA-DQB106:02. The meta-analysis revealed that HLA-DQB104:01, HLA-DQB105:02, HLA-DQB105:03, and HLA-DQB106:01 were risk factors for HBV infection susceptibility, while HLA-DQB105:01, HLA-DQB106:03, and HLA-DQB106:04 protected against HBV infection. Spontaneous HBV clearance was associated withHLA-DQB106:04, while chronic HBV infection was associated with HLA-DQB102:01 and HLA-DQB1*05:02.
DBQ1 typing can be used to identify patients who have elevated risks of HBV infection (i.e., patients with HLA-DQB104:01, HLA-DQB105:02, HLA-DQB105:03, and HLA-DQB106:01) or elevated risks of chronic HBV infection (i.e., patients with HLA-DQB102:01 and HLA-DQB105:02).
乙型肝炎病毒（HBV）感染是肝硬化和肝细胞癌的独立危险因素，HLA-DQB1 多态性在 HBV 感染中发挥重要作用。
本研究检测了 256 例 HBV 携带者和 433 例健康对照者中 HLA-DQB1 等位基因与 HBV 感染易感性之间的关系。采集静脉血进行 DQB1 高分辨率分型，并检测干扰素-γ、白细胞介素-4（IL-4）、白细胞介素-10 和 DQB1mRNA 表达。还对评估 HLA-DQB1 等位基因与 HBV 感染和清除相关性的相关病例对照研究进行了荟萃分析。
我们发现 HLA-DQB106:03 可预防 HBV 感染。与 HLA-DQB105:03 相比，HBV 病例中 HLA-DQB106:05 时 IFN-γ 和 IL-4 水平显著升高，且与 HLA-DQB105:03 和 HLA-DQB106:02 相比，HBV 组的 DQB1mRNA 表达更高。荟萃分析显示，HLA-DQB104:01、HLA-DQB105:02、HLA-DQB105:03 和 HLA-DQB106:01 是 HBV 感染易感性的危险因素，而 HLA-DQB105:01、HLA-DQB106:03 和 HLA-DQB106:04 则可预防 HBV 感染。HBV 自发清除与 HLA-DQB106:04 相关，而慢性 HBV 感染与 HLA-DQB102:01 和 HLA-DQB1*05:02 相关。
DBQ1 分型可用于识别 HBV 感染风险增加（即 HLA-DQB104:01、HLA-DQB105:02、HLA-DQB105:03 和 HLA-DQB106:01）或慢性 HBV 感染风险增加（即 HLA-DQB102:01 和 HLA-DQB105:02）的患者。
