Department of Laboratory Medicine, West China Second University Hospital, Chengdu, Sichuan, China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, China.
Immunogenetics. 2021 Jun;73(3):253-261. doi: 10.1007/s00251-021-01213-w. Epub 2021 Mar 12.
Hepatitis B virus (HBV) affects approximately 68 million people in China, and 10-15% of adults infected with HBV develop chronic hepatitis B, liver cirrhosis, liver failure or hepatocellular carcinoma (HCC). HLA-DPB1 gene polymorphism and expression have been shown to be associated with HBV infection susceptibility and spontaneous clearance. The aim of this study is to evaluate the role of HLA-DPB1 gene polymorphism in HBV infection. HLA-DPB1 and rs9277535 polymorphisms were investigated in 259 patients with HBV infection and 442 healthy controls (HCs) using sequence-based typing. The mRNA of HLA-DPB1 was measured by real-time polymerase chain reaction. HLA-DPB1 genes and rs9277535 polymorphisms were all associated with HBV infection in the Sichuan Han population. rs9277535A and HLA-DPB104:02 played a protective role against HBV infection. rs9277535G and DPB105:01 were associated with susceptibility to HBV infection. rs9277535GG had significantly higher HLA-DPB1 mRNA expression in the HBV infection group compared with the HC group. HLA-DPB105:01 and HLA-DPB121:01 had significantly lower mRNA expression in the HBV infection group compared with the HC group. The meta-analysis revealed that HLA-DPB102:01, HLA-DPB102:02, HAL-DPB104:01 and HLA-DPB104:02 protected against HBV infection, while HLA-DPB105:01, HLA-DPB109:01, and HLA-DPB113:01 were risk factors for susceptibility to HBV infection. HLA-DPB102:01, HLA-DPB102:02, and HLA-DPB104:01 were associated with HBV spontaneous clearance, while HLA-DPB1*05:01 was associated with chronic HBV infection. HLA-DPB1 alleles and rs9277535 have a major effect on the risk of HBV infection, and HBV infection is associated with lower HLA-DPB1 expression. HLA-DPB1 alleles have an important role in HBV susceptibility and spontaneous clearance.
乙型肝炎病毒（HBV）影响中国约 6800 万人，10-15%的 HBV 感染者发展为慢性乙型肝炎、肝硬化、肝功能衰竭或肝细胞癌（HCC）。HLA-DPB1 基因多态性和表达已被证明与 HBV 感染易感性和自发性清除有关。本研究旨在评估 HLA-DPB1 基因多态性在 HBV 感染中的作用。采用基于序列的分型方法，对 259 例 HBV 感染患者和 442 例健康对照（HC）进行 HLA-DPB1 和 rs9277535 多态性检测。采用实时聚合酶链反应检测 HLA-DPB1mRNA。在四川汉族人群中，HLA-DPB1 基因和 rs9277535 多态性均与 HBV 感染相关。rs9277535A 和 HLA-DPB104:02 对 HBV 感染有保护作用。rs9277535G 和 DPB105:01 与 HBV 感染易感性相关。与 HC 组相比，HBV 感染组 rs9277535GG 具有显著较高的 HLA-DPB1mRNA 表达。与 HC 组相比，HBV 感染组 HLA-DPB105:01 和 HLA-DPB121:01 的 mRNA 表达显著降低。荟萃分析显示，HLA-DPB102:01、HLA-DPB102:02、HAL-DPB104:01 和 HLA-DPB104:02 可预防 HBV 感染，而 HLA-DPB105:01、HLA-DPB109:01 和 HLA-DPB113:01 是 HBV 感染易感性的危险因素。HLA-DPB102:01、HLA-DPB102:02 和 HLA-DPB104:01 与 HBV 自发性清除有关，而 HLA-DPB1*05:01 与慢性 HBV 感染有关。HLA-DPB1 等位基因和 rs9277535 对 HBV 感染风险有重要影响，HBV 感染与 HLA-DPB1 表达降低有关。HLA-DPB1 等位基因在 HBV 易感性和自发性清除中起重要作用。
