Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, No. 11 Zhongguancun Beiyitiao, Beijing, 100190, P. R. China.
Chem Soc Rev. 2019 Feb 4;48(3):850-884. doi: 10.1039/c8cs00303c.
Barcode technology can deliver batched information for patient healthcare. For clinical examinations, barcodes serve as reporters for labeling multiple targets, and meanwhile, facilitate improved sensitivity and specificity, thus enabling barcode as a promising alternative to traditional labels for biomarker identification and signal amplification. However, faced with the stringent claims of point-of-care (POC) bioassays, efforts are needed to advance current technologies toward rapidity, robustness, affordability, and user-friendliness. In the past decades, chemists have succeeded in delicate fabrication of the barcode libraries for encoding. Nevertheless, the decoding technologies remain poorly discussed, especially simplified decoding strategies for POC bioassays. Recent emergence of portable cartridges and miniaturized signal-recording devices has brought a promise to merge barcodes-assisted bioassay with POC testing (POCT). This review provides a comprehensive summary on barcode encoding and decoding, with emphasis on their potential use in POCT, facilitated by improved manufacturing and portable devices. Future directions of barcoded bioassays for POCT and current challenges are also presented. We anticipate that this review will be beneficial to promoting barcodes toward broad applications.
条码技术可提供患者医疗保健的批量信息。在临床检查中，条码可作为标记多个目标的报告器，同时提高了灵敏度和特异性，因此条码有望成为传统标签用于生物标志物识别和信号放大的替代品。然而，面对即时护理（POC）生物分析的严格要求，需要努力推进当前技术的快速性、稳健性、可负担性和用户友好性。在过去的几十年中，化学家们已经成功地精细制造了用于编码的条码库。然而，解码技术仍未得到充分讨论，特别是用于 POC 生物分析的简化解码策略。最近便携式盒式磁带和小型化信号记录设备的出现带来了将条码辅助生物分析与即时检测（POCT）相结合的希望。本综述全面总结了条码的编码和解码，重点介绍了通过改进制造和便携式设备，条码在 POCT 中的潜在应用。还提出了用于 POCT 的条码生物分析的未来方向和当前挑战。我们预计，这篇综述将有助于推动条码的广泛应用。
