Yamaguchi R, Matsuo K, Yamazaki A, Nagai S, Terasaka K, Yamada T
Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan.
FEBS Lett. 1988 Nov 21;240(1-2):115-7. doi: 10.1016/0014-5793(88)80350-8.
Using a single-probe method, we have cloned the gene for an immunogenic MPB57 protein of Mycobacterium bovis BCG. The nucleotide sequence includes an ORF of 300 base pairs encoding a protein of 99 amino acids with an Mr of 10,818. This cloned gene was expressed in an Escherichia coli expression vector to give a mature protein which reacted with a polyclonal antibody raised against MPB57.
我们采用单探针法克隆了卡介苗（Mycobacterium bovis BCG）免疫原性MPB57蛋白的基因。核苷酸序列包含一个300个碱基对的开放阅读框，编码一个由99个氨基酸组成、分子量为10,818的蛋白质。该克隆基因在大肠杆菌表达载体中表达，产生一种能与抗MPB57多克隆抗体发生反应的成熟蛋白。
