Matsumoto S, Matsuo T, Ohara N, Hotokezaka H, Naito M, Minami J, Yamada T
Nagasaki University, School of Dentistry, Japan.
Scand J Immunol. 1995 Mar;41(3):281-7. doi: 10.1111/j.1365-3083.1995.tb03565.x.
MPB70 is known to be an immunogenic mycobacterial protein secreted in large amounts from Mycobacterium bovis BCG (BCG) Tokyo. The analogous gene for MPT70 was cloned from Mycobacterium tuberculosis H37Rv which produces this protein in only a small amount. The gene encoding 193 amino acid residues including 30 amino acids for the signal peptide, the promoter-like sequence, and the ribosome-binding site, was completely identical to that of BCG Tokyo. Computer analysis revealed that the carboxy-terminal half of MPT70 was homologous to amino acid sequences of fasciclin I, osteoblast-specific factor 2 (OSF-2), and human transforming growth factor-beta induced gene product (beta IG-H3). Escherichia coli (E. coli) -mycobacteria shuttle vectors containing mpt70 or mpb70 genes 0.7kbp upstream of the 5' end of them were able to be expressed in BCG Pasteur which is a MPB70 low-producer, but the extent of the expression was not that of a high-producer.
已知MPB70是一种具有免疫原性的分枝杆菌蛋白，由卡介苗（BCG）东京株大量分泌。MPT70的类似基因是从结核分枝杆菌H37Rv中克隆出来的，该菌株仅少量产生这种蛋白质。编码193个氨基酸残基（包括30个氨基酸的信号肽、启动子样序列和核糖体结合位点）的基因与卡介苗东京株的基因完全相同。计算机分析表明，MPT70的羧基末端一半与成束蛋白I、成骨细胞特异性因子2（OSF-2）和人转化生长因子β诱导基因产物（βIG-H3）的氨基酸序列同源。含有mpt70或mpb70基因（位于其5'端上游0.7kbp处）的大肠杆菌-分枝杆菌穿梭载体能够在MPB70低产的卡介苗巴斯德株中表达，但表达程度不如高产株。
