Soghaier Mohammed A, Abdelgadir Deena M, Abdelkhalig Sozan M, Kafi Hamoda, Zarroug Isam M A, Sall Amadou A, Eldegai Mawahib H, Elageb Rehab M, Osman Muntasir M, Khogali Hayat
Epidemiology and Zoonotic Diseases Department, Federal Ministry of Health, Osman Digna Street with Nile Avenue, PO Box 303, 1111, Khartoum, Sudan.
The Department of Epidemiology, National Public Health Laboratory, Federal Ministry of Heath, Khartoum, Sudan.
BMC Res Notes. 2018 Dec 19;11(1):906. doi: 10.1186/s13104-018-4027-9.
The purpose of this study is to provide the first evidence of Zika virus circulation (ZIK) in Sudan. Zika virus was first isolated in the Zika forest of Uganda in 1947, and in 2016, the World Health Assembly declared it a public health emergency of international concern. The discovery of Zika virus circulation in Sudan came as a secondary finding in a 2012 country-wide yellow fever prevalence study, when laboratory tests were done to exclude cross-reactions between flaviviruses. The study was cross-sectional community-based, with randomly selected participants through multi-stage cluster sampling. A sub-set of samples were tested for the Zika virus using ELISA, and the ones that demonstrated reactive results were subsequently tested by PRNT.
The prevalence of Zika IgG antibodies among ELISA-tested samples was 62.7% (59.4 to 66.1, 95% CI), and only one sample was found positive when tested by PRNT. This provided the first documented evidence for the pre-existing circulation of Zika virus circulation in Sudan. This evidence provides the foundation for future research in this field, and further structured studies should be conducted to determine the epidemiology and burden of the disease.
本研究旨在提供苏丹存在寨卡病毒传播（ZIK）的首个证据。寨卡病毒于1947年首次在乌干达的寨卡森林中分离出来，2016年，世界卫生大会宣布其为国际关注的突发公共卫生事件。苏丹寨卡病毒传播的发现是在2012年全国黄热病流行率研究中的一个次要发现，当时进行实验室检测以排除黄病毒之间的交叉反应。该研究是以社区为基础的横断面研究，通过多阶段整群抽样随机选择参与者。使用酶联免疫吸附测定（ELISA）对一部分样本进行寨卡病毒检测，对显示有反应性结果的样本随后通过空斑减少中和试验（PRNT）进行检测。
在接受ELISA检测的样本中，寨卡病毒IgG抗体的流行率为62.7%（59.4至66.1，95%置信区间），通过PRNT检测时仅发现一个样本呈阳性。这为苏丹此前存在寨卡病毒传播提供了首个文献记载的证据。这一证据为该领域未来的研究奠定了基础，应开展进一步的结构化研究以确定该疾病的流行病学和负担情况。
