Gong Bin, Chen Shuang, Lan Wenwen, Huang Yanmin, Zhu Xiangcheng
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Ocean Science department, Qinzhou University, Qinzhou, China.
College of Chemistry and Materials Science, Guangxi Teachers Education University, Nanning, China.
Iran J Pharm Res. 2018 Fall;17(4):1339-1346.
Mangroves are the tidal forest existing in the intertidal zone and usually considered as the special marine ecosystem. In the present study, 452 actinomycetes were recovered from nine diferent sites at Maowei Sea Mangrove Reserve in Qinzhou (Guangxi province, China). Among them, Seventy-four strains were purified for 16s RNA gene sequencing and further characterization. The results indicated that the majority of isolates belonged to the genera including 17 species. was the dominant species (31.1%), followed by (17.5%), (10.8%) and other species. Only one rare actinomycete, was discovered The isolation of actinomycetes was obviously related to the type of soil and edaphic conditions. Rhizosphere-associated soils gave almost 62.2% actinomycete isolates, nearly twice as many as the non-rhizosphere-associated soils. In addition, 20 actinomycete strains (27%) presented varied antibacterial activities towards four tested organisms, including two drug-resistant clinical strains (MRSA and VRE), while some species of like , , , , and exhibited remarkable cytotoxic activities. Further bioinformatical analysis of these 29 bioactive strains for secondary metabolites biosynthetic machineries revealed that nonribosomal peptide synthetase (NRPS) was detected in 20 isolates (68.9%), whereas type-I polyketide synthase (PKS-I) and type-II polyketide synthase (PKS-II) were detected in 16 and all of the 29 strains, respectively. Hence, our work demonstrated that actinomycetes from mangroves in Maowei Sea Mangrove Reservewere fascinating reservoirs for antibacterial and antitumor natural products discovery.
红树林是存在于潮间带的潮汐森林，通常被视为特殊的海洋生态系统。在本研究中，从钦州茅尾海红树林保护区（中国广西省）的九个不同地点分离出452株放线菌。其中，74株被纯化用于16s RNA基因测序和进一步表征。结果表明，大多数分离株属于多个属，包括17个物种。 是优势种（31.1%），其次是 （17.5%）、 （10.8%）和其他物种。仅发现一种稀有放线菌 。放线菌的分离明显与土壤类型和土壤条件有关。根际相关土壤中放线菌分离株占近62.2%，几乎是非根际相关土壤的两倍。此外，20株放线菌菌株（27%）对四种受试生物表现出不同的抗菌活性，包括两种耐药临床菌株（耐甲氧西林金黄色葡萄球菌和耐万古霉素肠球菌），而某些 属的物种，如 、 、 、 、 和 表现出显著的细胞毒性活性。对这29株生物活性菌株的次生代谢物生物合成机制进行的进一步生物信息学分析表明，20株分离株（68.9%）中检测到非核糖体肽合成酶（NRPS），而在16株和所有29株菌株中分别检测到I型聚酮合酶（PKS-I）和II型聚酮合酶（PKS-II）。因此，我们的工作表明，茅尾海红树林保护区红树林中的放线菌是发现抗菌和抗肿瘤天然产物的迷人资源。
