Alipoor A, Fardid R, Sharifzadeh S
M.Sc. Radiology Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Associate Professor, Radiology Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
J Biomed Phys Eng. 2018 Dec 1;8(4):393-402. eCollection 2018 Dec.
Coronary heart disease (CHD) is one of the most common diseases. Coronary angiography (CAG) is an important apparatus used to diagnose and treat this disease. Since angiography is performed through exposure to ionizing radiation, it can cause harmful effects induced by double-stranded breaks in DNA which is potentially life-threatening damage. The aim of the present study is to investigate phosphorylation of Histone H2AX in the location of double-stranded breaks in peripheral blood lymphocytes as an indication of biological effects of radiation on angiography.
This method is based on the phosphorylation measurement of Histone (gamma-H2AX or γ-H2AX) levels on serine 139 after the formation of DNA double-strand break. 5 cc of blood samples from 24 patients undergoing angiography were taken pre- and post-radiation. Blood lymphocytes were extracted, fixed and stained with specific γ-H2AX antibodies. Finally, the percentage of phosphorylation of Histone H2AX as an indicator of double-strand break was measured by a cytometry technique.
An increase was observed in all patients' percentage of phosphorylated Histone H2AX (double-stranded breaks DNA) after radiation (20.15 ± 14.18) compared to pre-exposure time (1.52 ± 0.34). Also, the mean of DNA double-strand break is shown in a linear correlation with DAP.
Although induction of DNA double-strand breaks was associated with the radiation dose in patients, the effect of individual factors such as radio-sensitivity and regenerative capacity should not be ignored. In the future, if we are able to measure DNA damage response in every angiography patient, we will use it as a biomarker for the patient dose; this will promote public health.
Using flow cytometers readings done automatically is possible to detect γ-H2AX in the number of blood cells, therefore, the use of this technique could play a significant role in monitoring patients.
冠心病（CHD）是最常见的疾病之一。冠状动脉造影（CAG）是用于诊断和治疗该疾病的重要手段。由于血管造影是通过暴露于电离辐射进行的，它会导致DNA双链断裂引起的有害影响，这是潜在的危及生命的损伤。本研究的目的是调查外周血淋巴细胞双链断裂部位组蛋白H2AX的磷酸化情况，以此作为血管造影辐射生物学效应的指标。
该方法基于DNA双链断裂形成后丝氨酸139位点组蛋白（γ-H2AX）水平的磷酸化测量。采集24例接受血管造影患者辐射前后各5毫升血样。提取血液淋巴细胞，固定并用特异性γ-H2AX抗体染色。最后，通过细胞计数技术测量组蛋白H2AX磷酸化百分比作为双链断裂的指标。
与辐射前时间（1.52±0.34）相比，所有患者辐射后组蛋白H2AX磷酸化百分比（双链断裂DNA）均有所增加（20.15±14.18）。此外，DNA双链断裂平均值与剂量面积乘积呈线性相关。
虽然患者DNA双链断裂的诱导与辐射剂量有关，但个体因素如放射敏感性和再生能力的影响也不容忽视。未来，如果我们能够在每位血管造影患者中测量DNA损伤反应，我们将把它用作患者剂量的生物标志物；这将促进公众健康。
使用自动完成的流式细胞仪读数能够检测血细胞数量中的γ-H2AX，因此，这项技术的应用在监测患者方面可能发挥重要作用。
