Military Medical Research Center, Bucharest, Romania.
National Institute for Laser Plasma and Radiation Physics, Bucharest, Romania.
Health Phys. 2020 Jul;119(1):101-108. doi: 10.1097/HP.0000000000001267.
Ionizing radiation exposure produces direct or indirect biological effects on genomic DNA. The latter are ionizing radiation mediated by induction of free radicals and oxygen species (ROS). The study was conducted to evaluate the dose-effect/time-effect of antioxidant treatments in reducing the induction of double-strand breaks in human blood lymphocytes. Human peripheral blood samples of 2 mL each from healthy donors were irradiated with 10 mGy after pre-incubation with different antioxidants (β-carotene, vitamin E, vitamin C, N-acetyl L-cysteine). In order to assess their efficiency as prophylactic therapy for irradiation, various concentrations and combinations of antioxidants, as well as different incubation times, have been evaluated. To assess double-strand breaks induced by ionizing radiation, the phosphorylated histone γ-H2AX has been used. A significant reduction (p < 0.001) in double-strand breaks studied with a γ-H2AX assay was observed with N-acetyl L-cysteine with a 1-h incubation time, followed by vitamin C, vitamin E, and β-carotene. The use of antioxidants, especially N-acetyl L-cysteine before irradiation, significantly decreased the occurrence of double-strand breaks, demonstrating the potential radiological protection for exposure to ionizing radiation.
电离辐射会对基因组 DNA 产生直接或间接的生物学效应。后者是由自由基和氧自由基（ROS）诱导的电离辐射介导的。本研究旨在评估抗氧化剂处理在减少人血淋巴细胞双链断裂诱导方面的剂量-效应/时间-效应。将来自健康供体的 2 mL 人外周血样本在预孵育不同抗氧化剂（β-胡萝卜素、维生素 E、维生素 C、N-乙酰-L-半胱氨酸）后用 10 mGy 照射。为了评估它们作为预防照射的治疗方法的效率，已经评估了各种浓度和组合的抗氧化剂以及不同的孵育时间。为了评估电离辐射诱导的双链断裂，使用了磷酸化组蛋白 γ-H2AX。用 γ-H2AX 测定法研究的双链断裂明显减少（p < 0.001），用 1 小时孵育时间的 N-乙酰-L-半胱氨酸，其次是维生素 C、维生素 E 和 β-胡萝卜素。在照射前使用抗氧化剂，特别是 N-乙酰-L-半胱氨酸，可显著降低双链断裂的发生，表明其对电离辐射暴露具有潜在的放射防护作用。
