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EnvZ是大肠杆菌K-12的一种跨膜环境传感器，可在体外被磷酸化。

EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitro.

作者信息

Igo M M, Silhavy T J

机构信息

Department of Molecular Biology, Princeton University, New Jersey 08544.

出版信息

J Bacteriol. 1988 Dec;170(12):5971-3. doi: 10.1128/jb.170.12.5971-5973.1988.

DOI:10.1128/jb.170.12.5971-5973.1988
PMID:3056929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211718/
Abstract

By fusing the transcriptional and translational start signals of lacZ to envZ, we have obtained high-level synthesis of a truncated EnvZ protein (EnvZ115) in which the first 38 amino acids of EnvZ are replaced with the first 8 amino acids of LacZ. Using this construct, we have partially purified the EnvZ115 protein and demonstrated that this protein can be phosphorylated in vitro. We suggest that phosphorylation may be an important feature of EnvZ function.

摘要

通过将lacZ的转录和翻译起始信号与envZ融合，我们获得了截短的EnvZ蛋白（EnvZ115）的高水平合成，其中EnvZ的前38个氨基酸被LacZ的前8个氨基酸所取代。利用这个构建体，我们部分纯化了EnvZ115蛋白，并证明该蛋白在体外可以被磷酸化。我们认为磷酸化可能是EnvZ功能的一个重要特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/5291d05b198b/jbacter00190-0580-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/d86369bbbbb7/jbacter00190-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/3de0a3c06373/jbacter00190-0580-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/0a6a436ff632/jbacter00190-0580-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/5291d05b198b/jbacter00190-0580-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/d86369bbbbb7/jbacter00190-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/3de0a3c06373/jbacter00190-0580-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/0a6a436ff632/jbacter00190-0580-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20dd/211718/5291d05b198b/jbacter00190-0580-d.jpg

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EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitro.EnvZ是大肠杆菌K-12的一种跨膜环境传感器，可在体外被磷酸化。
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本文引用的文献

1
Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
2
Genetic analysis of the ompB locus in Escherichia coli K-12.大肠杆菌K-12中ompB基因座的遗传分析。
J Mol Biol. 1981 Sep 5;151(1):1-15. doi: 10.1016/0022-2836(81)90218-7.
3
Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product.基因表达的渗透调节。II. 大肠杆菌ompB操纵子envZ基因的DNA序列及其基因产物的特性
Front Microbiol. 2018 Oct 12;9:2405. doi: 10.3389/fmicb.2018.02405. eCollection 2018.
4
An Overview of Two-Component Signal Transduction Systems Implicated in Extra-Intestinal Pathogenic Infections.与肠道外致病性感染相关的双组分信号转导系统概述
Front Cell Infect Microbiol. 2017 May 9;7:162. doi: 10.3389/fcimb.2017.00162. eCollection 2017.
5
An Two-Component System Ortholog Regulates Phase Variation, Osmotic Tolerance, Motility, and Virulence in Acinetobacter baumannii Strain AB5075.一种双组分系统直系同源物调控鲍曼不动杆菌AB5075菌株的相变、渗透压耐受性、运动性和毒力。
J Bacteriol. 2017 Jan 12;199(3). doi: 10.1128/JB.00705-16. Print 2017 Feb 1.
6
Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS.IV型菌毛蛋白通过与传感激酶PilS的直接膜内相互作用来调节自身表达。
Proc Natl Acad Sci U S A. 2016 May 24;113(21):6017-22. doi: 10.1073/pnas.1512947113. Epub 2016 May 9.
7
The importance of the viable but non-culturable state in human bacterial pathogens.可存活但不可培养状态在人类细菌病原体中的重要性。
Front Microbiol. 2014 Jun 2;5:258. doi: 10.3389/fmicb.2014.00258. eCollection 2014.
8
Comprehensive analysis of OmpR phosphorylation, dimerization, and DNA binding supports a canonical model for activation.全面分析 OmpR 的磷酸化、二聚化和 DNA 结合，支持激活的经典模型。
J Mol Biol. 2013 May 27;425(10):1612-26. doi: 10.1016/j.jmb.2013.02.003. Epub 2013 Feb 8.
9
OmpR regulation of the uropathogenic Escherichia coli fimB gene in an acidic/high osmolality environment.酸性/高渗透压环境中 OmpR 对尿路致病性大肠杆菌 fimB 基因的调控。
Microbiology (Reading). 2013 Feb;159(Pt 2):316-327. doi: 10.1099/mic.0.059386-0. Epub 2012 Nov 22.
10
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J Biol Chem. 1982 Nov 25;257(22):13692-8.
4
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Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
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Cell. 1987 Jun 5;49(5):579-81. doi: 10.1016/0092-8674(87)90530-7.
6
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J Biol Chem. 1986 Nov 15;261(32):15252-6.
7
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J Biol Chem. 1986 Nov 15;261(32):15075-80.
8
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9
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J Biochem. 1987 Feb;101(2):387-96. doi: 10.1093/oxfordjournals.jbchem.a121923.
10
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Proc Natl Acad Sci U S A. 1988 Aug;85(15):5492-6. doi: 10.1073/pnas.85.15.5492.