Hess J F, Oosawa K, Matsumura P, Simon M I
Division of Biology, California Institute of Technology, Pasadena 91125.
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7609-13. doi: 10.1073/pnas.84.21.7609.
The nature of the biochemical signal that is involved in the excitation response in bacterial chemotaxis is not known. However, ATP is required for chemotaxis. We have purified all of the proteins involved in signal transduction and show that the product of the cheA gene is rapidly autophosphorylated, while some mutant CheA proteins cannot be phosphorylated. The presence of stoichiometric levels of two other purified components in the chemotaxis system, the CheY and CheZ proteins, induces dephosphorylation. We suggest that the phosphorylation of CheA by ATP plays a central role in signal transduction in chemotaxis.
参与细菌趋化性兴奋反应的生化信号的本质尚不清楚。然而，趋化性需要ATP。我们已经纯化了所有参与信号转导的蛋白质，并表明cheA基因的产物会迅速自磷酸化，而一些突变的CheA蛋白则不能被磷酸化。趋化性系统中另外两种纯化成分（CheY和CheZ蛋白）的化学计量水平的存在会诱导去磷酸化。我们认为，ATP介导的CheA磷酸化在趋化性信号转导中起核心作用。
