Institute for Grapevine Breeding Geilweilerhof, Julius Kuehn Institute, Federal Research Centre of Cultivated Plants, Geilweilerhof, 76833, Siebeldingen, Germany.
Institute for Crop and Soil Science, Julius Kuehn Institute, Federal Research Centre of Cultivated Plants, Bundesallee 58, 38116, Brunswick, Germany.
Theor Appl Genet. 2019 Apr;132(4):1159-1177. doi: 10.1007/s00122-018-3269-1. Epub 2018 Dec 19.
Loose cluster architecture is an important aim in grapevine breeding since it has high impact on the phytosanitary status of grapes. This investigation analyzed the contributions of individual cluster sub-traits to the overall trait of cluster architecture. Six sub-traits showed large impact on cluster architecture as major determinants. They explained 57% of the OIV204 descriptor for cluster compactness rating in a highly diverse cross-population of 149 genotypes. Genetic analysis revealed several genomic regions involved in the expression of this trait. Based on the linkage of phenotypic features to molecular markers, QTL calculations shed new light on the genetic determinants of cluster architecture. Eight QTL clusters harbor overlapping confidence intervals of up to four co-located QTLs. A physical projection of the QTL clusters by confidence interval-flanking markers onto the PN40024 reference genome sequence revealed genes enriched in these regions.
松散的集群结构是葡萄育种的一个重要目标，因为它对葡萄的植物卫生状况有很大的影响。本研究分析了单个集群子特征对集群结构整体特征的贡献。六个子特征作为主要决定因素对集群结构有很大的影响。它们在一个由 149 个基因型组成的高度多样化的杂交群体中，解释了 OIV204 描述符中关于集群紧凑度评分的 57%。遗传分析揭示了参与该性状表达的几个基因组区域。基于表型特征与分子标记的连锁，QTL 计算为集群结构的遗传决定因素提供了新的认识。八个 QTL 簇含有多达四个共定位 QTL 的重叠置信区间。通过置信区间侧翼标记将 QTL 簇在 PN40024 参考基因组序列上进行物理投影，揭示了这些区域中富集的基因。
