Lee Inae, Kim Hae-Yeong
Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Micromachines (Basel). 2024 Dec 21;15(12):1524. doi: 10.3390/mi15121524.
Lab-on-a-chip (LOC) devices have been developed for nucleic acid analysis by integrating complex laboratory functions onto a miniaturized chip, enabling rapid, cost-effective, and highly sensitive on-site testing. This review examines the application of LOC technology in food safety, specifically in the context of nucleic acid-based analyses for detecting pathogens and contaminants. We focus on microfluidic-based LOC devices that optimize nucleic acid extraction and purification on the chip or amplification and detection processes based on isothermal amplification and polymerase chain reaction. We also explore advancements in integrated LOC devices that combine nucleic acid extraction, amplification, and detection processes within a single chip to minimize sample preparation time and enhance testing accuracy. The review concludes with insights into future trends, particularly the development of portable LOC technologies for rapid and efficient nucleic acid testing in food safety.
芯片实验室（LOC）设备已被开发用于核酸分析，它将复杂的实验室功能集成到一个小型化芯片上，能够实现快速、经济高效且高度灵敏的现场检测。本综述探讨了LOC技术在食品安全中的应用，特别是在基于核酸分析检测病原体和污染物方面的应用。我们重点关注基于微流控的LOC设备，这些设备可在芯片上优化核酸提取和纯化，或基于等温扩增和聚合酶链反应进行扩增和检测过程。我们还探讨了集成LOC设备的进展，这些设备在单个芯片内结合了核酸提取、扩增和检测过程，以最大限度地减少样品制备时间并提高检测准确性。综述最后对未来趋势进行了展望，特别是开发用于食品安全中快速高效核酸检测的便携式LOC技术。
