Huang Tianzeng, Li Linzhi, Liu Xing, Chen Qi, Fang Xueen, Kong Jilie, Draz Mohamed S, Cao Hongmei
College of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China.
Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P. R. China.
Anal Methods. 2020 Dec 7;12(46):5551-5561. doi: 10.1039/d0ay01768j.
Food safety is a major and enduring challenge and has a profound impact on the quality of human life. Loop-mediated isothermal amplification (LAMP) is a relatively novel gene amplification method under isothermal conditions with rapidity, simplicity and high specificity. This review will describe the principles and development of the LAMP technique along with its advantages and disadvantages, such as LAMP integrated on classical microfluidic chips, paper-chips, electrochemical devices, nanomaterials and digital devices. Moreover, we will systematically and comprehensively review its applications in the field of food safety, such as in the detection of foodborne pathogens, allergens and organophosphorus pesticides and genetically modified organisms; finally its development trends in food safety will be discussed.
食品安全是一项重大且持久的挑战，对人类生活质量有着深远影响。环介导等温扩增技术（LAMP）是一种相对新颖的等温基因扩增方法，具有快速、简便和高特异性的特点。本文综述将阐述LAMP技术的原理、发展历程及其优缺点，例如集成在传统微流控芯片、纸芯片、电化学装置、纳米材料和数字设备上的LAMP技术。此外，我们将系统全面地综述其在食品安全领域的应用，如食源性病原体、过敏原、有机磷农药及转基因生物的检测；最后探讨其在食品安全方面的发展趋势。
