Department of Biotechnology, University of Calcutta, 673635, Kerala, India.
Curr Top Med Chem. 2018;18(29):2502-2510. doi: 10.2174/1568026619666181220110038.
Fabaceae are a rich source of protease inhibitors.
A proteinaceous protease inhibitor of 25 KDa designated as Macrotyloma uniflorum protease inhibitor (MUPI) was isolated from seeds of Macrotyloma uniflorum and purified to homogeneity by ammonium sulphate precipitation, DEAE Sepharose column and CNBr activated Sepharose 4B Trypsin affinity chromatography. The purity was checked by reverse phase HPLC. Long-term type 2 diabetes can lead to various biological complications, such as hypertension and heart-related diseases. The glucose uptake studies were carried out with the purified MUPI and it reveals its potential to be explored as a potent anti-hyperglycemic agent. MUPI was rendered safer on HepG2 cells after MTT cytotoxicity assay.
The results of glucose uptake studies suggest MUPI to be equally efficient to that of the positive control drug Metformin against diabetes mellitus.
The significance of plant-based inhibitors for modulating carbohydrate breakdown and control of glycemic index is explored to reduce the risk factors and side effects of the available drugs.
豆科植物是蛋白酶抑制剂的丰富来源。
从豇豆种子中分离出一种 25 kDa 的蛋白蛋白酶抑制剂，命名为豇豆胰蛋白酶抑制剂（MUPI），并通过硫酸铵沉淀、DEAE Sepharose 柱和 CNBr 活化 Sepharose 4B 胰蛋白酶亲和层析进行纯化至均一性。通过反相 HPLC 检查纯度。长期 2 型糖尿病会导致各种生物并发症，如高血压和心脏相关疾病。用纯化的 MUPI 进行葡萄糖摄取研究，结果表明它具有作为潜在抗高血糖药物的潜力。MTT 细胞毒性测定后，MUPI 在 HepG2 细胞上变得更安全。
葡萄糖摄取研究的结果表明，MUPI 对抗糖尿病的效果与阳性对照药物二甲双胍一样有效。
探索基于植物的抑制剂在调节碳水化合物分解和控制血糖指数方面的意义，以降低现有药物的风险因素和副作用。
