Tse J, Goldfarb S
Department of Pathology, University of Wisconsin School of Medicine, Madison 53706.
J Histochem Cytochem. 1988 Dec;36(12):1527-31. doi: 10.1177/36.12.3057073.
We have developed a direct avidin-biotin-peroxidase complex (ABC) immunohistochemical method for localization of estrophilin in mouse tissues. The method has been found especially useful for microscopic demonstration of the receptor in mouse liver, since the indirect alternative, autoradiography after injection of radiolabeled estrogens, is of no value in this organ. The ABC technique employs a biotinylated monoclonal antibody to human estrophilin (Abbot H222) which was previously shown to crossreact with the murine receptor. Cryostat-cut tissue sections which were briefly fixed were incubated with the modified antibody, and the estrophilin was revealed by subsequent exposure to ABC followed by H2O2/diaminobenzidine.
我们开发了一种直接抗生物素蛋白-生物素-过氧化物酶复合物（ABC）免疫组织化学方法，用于在小鼠组织中定位雌激素受体。该方法已被证明对在小鼠肝脏中显微镜下显示该受体特别有用，因为间接方法，即注射放射性标记雌激素后的放射自显影，在这个器官中没有价值。ABC技术使用一种生物素化的抗人雌激素受体单克隆抗体（雅培H222），该抗体先前已被证明可与小鼠受体发生交叉反应。将经短暂固定的低温恒温器切片与修饰后的抗体孵育，随后通过暴露于ABC然后H2O2/二氨基联苯胺来显示雌激素受体。
