Press M F, Nousek-Goebl N A, Greene G L
J Histochem Cytochem. 1985 Sep;33(9):915-24. doi: 10.1177/33.9.4020102.
The recent production of a series of monoclonal estrophilin (estrogen receptor) antibodies recognizing estrogen receptor derived from a wide variety of animals and target tissues permits the development of immunoelectron microscopic techniques for identifying estrogen receptor. We have determined suitable conditions for the ultrastructural localization of estrogen receptor in tissue sections. Localization of receptor was observed in the euchromatin, but not in the marginated heterochromatin or nucleoli of epithelial and stromal nuclei of human endometrium. Competition studies indicate that only estrogen receptor specifically inhibits nuclear staining. The absence of any specific cytoplasmic localization at the electron-microscopic level is consistent with earlier light-microscopic observations and suggests that the majority of the cellular pool of estrophilin exists in the nucleus of hormone-responsive cells.
最近制备出了一系列单克隆雌激素亲和蛋白（雌激素受体）抗体，这些抗体可识别源自多种动物和靶组织的雌激素受体，这使得免疫电子显微镜技术得以发展，用于鉴定雌激素受体。我们已经确定了在组织切片中对雌激素受体进行超微结构定位的合适条件。在人类子宫内膜上皮和基质细胞核的常染色质中观察到了受体的定位，但在边缘化的异染色质或核仁中未观察到。竞争研究表明，只有雌激素受体能特异性抑制核染色。在电子显微镜水平上未发现任何特异性的细胞质定位，这与早期的光学显微镜观察结果一致，并表明雌激素亲和蛋白的大部分细胞池存在于激素反应性细胞的细胞核中。
