Czerkinsky C, Moldoveanu Z, Mestecky J, Nilsson L A, Ouchterlony O
Department of Microbiology, University of Alabama, Birmingham.
J Immunol Methods. 1988 Nov 25;115(1):31-7. doi: 10.1016/0022-1759(88)90306-7.
A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products. This variation permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products. Optimal conditions for the concurrent detection of human mononuclear cells secreting IgG or IgA antibodies are described.
已经开发出一种基于酶联免疫斑点法（ELISPOT）的新型检测系统，该系统采用两种产生不同颜色产物的免疫酶可视化系统的组合。这种变体允许同时计数分泌抗原性不同产物的两种不同类型的细胞。描述了同时检测分泌IgG或IgA抗体的人单核细胞的最佳条件。
