Tarkowski A, Czerkinsky C, Nilsson L A, Nygren H, Ouchterlony O
J Immunol Methods. 1984 Sep 4;72(2):451-9. doi: 10.1016/0022-1759(84)90013-9.
Although IgG rheumatoid factor may play an essential role in the pathogenesis of rheumatoid arthritis, there is no precise method for its specific detection at the cellular level. A modification of the recently developed enzyme-linked immunospot assay has been devised for enumeration of cells secreting IgG rheumatoid factor (IgG RF) and simultaneous quantitation of the IgG RF secreted. Specific, sensitive and simple, this new assay should provide a valuable tool for study of isotype-specific RF secretion by single cells.
尽管IgG类风湿因子可能在类风湿关节炎的发病机制中起关键作用，但在细胞水平上尚无对其进行特异性检测的精确方法。我们对最近开发的酶联免疫斑点试验进行了改进，用于计数分泌IgG类风湿因子（IgG RF）的细胞并同时定量所分泌的IgG RF。这种新试验特异性强、灵敏度高且操作简单，应为研究单细胞分泌的同型特异性RF提供一种有价值的工具。
