Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, Telangana, India.
Fisheries Research and Information Center (KVAFSU), Hesaraghatta Lake Post, Hesaraghatta, Bengaluru 560 089, India.
Gen Comp Endocrinol. 2019 Aug 1;279:67-77. doi: 10.1016/j.ygcen.2018.12.004. Epub 2018 Dec 17.
Common carp (Cyprinus carpio) is a world-wide freshwater fish of eutrophic waters. C. carpio, have various reproductive traits, including early sexual maturity, that may make them excellent, large, realistic, aquaculture model species. In the present work, de novo assembly of gonadal (testicular and ovarian) transcriptomes from juvenile common carp was performed to identify genes involved in gonadal development. A total of 81,757 and 43,257 transcripts with average lengths of 769 and 856 bp, were obtained from the immature testicular and ovarian transcriptomes, respectively. About 84,367 unigenes were constructed after removing redundancy involving representation of transcripts in both gonadal transcriptomes. Gene ontology (39,171 unigenes), clusters of orthologous group's analysis (6651 unigenes) and Kyoto encyclopedia of genes, and genomes automatic annotation server analysis (4783 unigenes) were performed to identify potential genes along with their functions. Furthermore, 18,342 (testis) and 8693 (ovary) simple sequence repeats were identified. About 298 differentially expressed genes were identified, of which 171 and 127 genes were up-regulated in testis and ovary, respectively. Quantitative real-time reverse transcription PCR was performed to validate differential expression of selected genes in testis and ovary. Nearly 809 genes related to reproduction were identified, sex-wise expression pattern of genes related to steroid synthesis, endocrine regulation, germ cell maintenance and others factors related to gonadal differentiation was observed, and expression analysis of nanos, ad4bp/sf-1, and gdf9 was performed. The present study identified certain important genes/factors involved in the gonadal development of C. carpio which may provide insights into the understanding of sex-differentiation and gonadal development processes.
鲤鱼（Cyprinus carpio）是一种广泛分布于富营养化水域的淡水鱼类。鲤鱼具有多种生殖特征，包括性早熟，这使它们成为优秀的、大型的、现实的水产养殖模式物种。在本研究中，我们对幼年鲤鱼的性腺（睾丸和卵巢）转录组进行从头组装，以鉴定参与性腺发育的基因。从未成熟的睾丸和卵巢转录组中分别获得了 81757 和 43257 条平均长度为 769 和 856bp 的转录本。去除两个性腺转录组中代表转录本的冗余后，共构建了 84367 个非冗余基因。对基因本体（39171 个非冗余基因）、直系同源群分析（6651 个非冗余基因）和京都基因与基因组百科全书自动注释服务器分析（4783 个非冗余基因）进行了分析，以确定潜在的基因及其功能。此外，还鉴定了 18342 个（睾丸）和 8693 个（卵巢）简单序列重复。鉴定了 298 个差异表达基因，其中 171 个和 127 个基因在睾丸和卵巢中分别上调。在睾丸和卵巢中进行了选定基因的定量实时逆转录 PCR 验证，结果显示，809 个与生殖相关的基因被鉴定出来，观察到与类固醇合成、内分泌调节、生殖细胞维持等因素相关的基因在性别上的表达模式，以及对 nanos、ad4bp/sf-1 和 gdf9 的表达分析。本研究鉴定了与鲤鱼性腺发育相关的某些重要基因/因子，这可能有助于深入了解性别分化和性腺发育过程。
