Belgian Ceramic Research Centre, Member of EMRA, Avenue Gouverneur Cornez, 4, B-7000 Mons, Belgium.
Belgian Ceramic Research Centre, Member of EMRA, Avenue Gouverneur Cornez, 4, B-7000 Mons, Belgium.
Mater Sci Eng C Mater Biol Appl. 2019 Feb 1;95:371-380. doi: 10.1016/j.msec.2018.03.004. Epub 2018 Mar 14.
The bioactivity of synthetic bone implants is highly impacted by their surface topography, especially by the presence of micro-patterns likely to generate cells growth guidance. In this study, laser machining technology was employed in order to produce controlled regular micro-patterns on dense calcium phosphate surfaces, without any contamination. The choice of the source was directed towards a femtosecond pulsed laser in order to limit the thermal impact of such a process and thus to avoid the unwanted phase transformations potentially induced by the temperature elevation. Beta tricalcium phosphate substrates with perfectly controlled micro-patterning and without any secondary phase were obtained by optimization of the process parameters (laser power, scanning speed, pulse frequency). The microstructural characteristics were investigated by microscopy (optical, confocal, scanning electron) and the phase identification was performed by X-ray diffraction. This work allowed highlighting the effects of the process parameters on the patterning. The high benefits of the laser treatment on wettability were shown by contact angle assays. Finally, the influence of surface micro-patterning on cell behavior was highlighted in vitro. This technique seems to provide an interesting alternative to conventional surface treatments of calcium phosphates.
合成骨植入物的生物活性受其表面形貌的影响很大，特别是受微图案的存在影响，微图案可能会产生细胞生长导向。在这项研究中，激光加工技术被用于在致密的磷酸钙表面上产生受控的规则微图案，而不会造成任何污染。选择这种激光加工技术是为了使用飞秒脉冲激光来限制这种工艺的热影响，从而避免因温度升高而可能引起的不必要的相变。通过优化工艺参数（激光功率、扫描速度、脉冲频率），获得了具有完美微图案且无任何副相的β-磷酸三钙基底。通过显微镜（光学显微镜、共聚焦显微镜、扫描电子显微镜）对微结构特征进行了研究，并通过 X 射线衍射进行了相鉴定。这项工作强调了工艺参数对图案化的影响。通过接触角测量法显示了激光处理对润湿性的高益处。最后，体外研究了表面微图案化对细胞行为的影响。这种技术似乎为传统的磷酸钙表面处理提供了一种很有前途的替代方法。
