Department of Materials Science and Engineering, The University of Tennessee, Knoxville, TN 37996, USA.
Biofabrication. 2010 Jun;2(2):025001. doi: 10.1088/1758-5082/2/2/025001. Epub 2010 Mar 10.
Calcium phosphate (Ca-P) coating on the Ti-6Al-4V alloy enhances osteoblast adhesion and tissue formation at the bone implant interface. In light of this, in the current work a laser-based coating technique was used to synthesize two different micro-textured (100 microm and 200 microm spaced line patterns) Ca-P coatings on the Ti-6Al-4V alloy and its effect on wettability and osteoblast cell adhesion were systematically studied. X-ray diffraction (XRD) analysis of the coated samples indicated the presence of precursor material, Ca10(PO4)6(OH)2 (HA) and various other additional phases such as CaTiO3, Ca3(PO4)2, TiO2 (anatase) and TiO2 (rutile) owing to the reaction between the precursor (HA) and substrate (Ti-6Al-4V) during laser processing. Confocal laser scanning microscopy-based characterization of coated samples indicated that the samples processed at 100 microm line spacing demonstrated a reduced surface roughness and smaller texture parameter value as compared to the samples processed at 200 microm spacing. The surface energy and wettability of the 100 microm spaced samples measured using a static sessile drop technique demonstrated higher surface energy and increased hydrophilicity as compared to the control (untreated Ti-6Al-4V) and the samples processed at 200 microm spacing. The tendency of coated samples for mineralization through generation of an apatite-like phase during immersion in a simulated body fluid was indicative of their in vitro bioactive nature. In light of higher surface energy and increased hydrophilicity the in vitro biocompatibility of the samples with 100 microm line spacing was demonstrated through increased cell proliferation and cell adhesion of mouse MC3T3-E1 osteoblast-like cells.
钙磷酸盐 (Ca-P) 涂层在 Ti-6Al-4V 合金上增强了成骨细胞在骨植入界面的黏附与组织形成。鉴于此，在当前工作中，采用基于激光的涂层技术在 Ti-6Al-4V 合金上合成了两种不同的微纹理（100 µm 和 200 µm 间隔的线图案）Ca-P 涂层，并系统研究了其对润湿性和成骨细胞黏附的影响。涂层样品的 X 射线衍射 (XRD) 分析表明，存在前驱体材料 Ca10(PO4)6(OH)2（HA）和其他各种附加相，如 CaTiO3、Ca3(PO4)2、TiO2（锐钛矿）和 TiO2（金红石），这是由于前驱体（HA）和基底（Ti-6Al-4V）之间在激光处理过程中的反应所致。基于共焦激光扫描显微镜的涂层样品表征表明，与处理线间距为 200 µm 的样品相比，处理线间距为 100 µm 的样品的表面粗糙度降低，纹理参数值更小。使用静态液滴技术测量的 100 µm 间隔样品的表面能和润湿性表明，与对照（未经处理的 Ti-6Al-4V）和处理线间距为 200 µm 的样品相比，具有更高的表面能和增加的亲水性。涂层样品在模拟体液中通过生成类磷灰石相而倾向于矿化，表明它们具有体外生物活性。鉴于更高的表面能和增加的亲水性，通过增加小鼠 MC3T3-E1 成骨样细胞的增殖和黏附，证明了具有 100 µm 线间距的样品具有体外生物相容性。
