Touya Nicolas, Al-Bourgol Samy, Désigaux Théo, Kérourédan Olivia, Gemini Laura, Kling Rainer, Devillard Raphaël
Univ. Bordeaux, INSERM, BIOTIS, U1026, F-33000 Bordeaux, France.
ALPhANOV, Rue François Mitterrand, 33400 Talence, France.
Bioengineering (Basel). 2023 Jan 24;10(2):155. doi: 10.3390/bioengineering10020155.
The laser patterning of implant materials for bone tissue engineering purposes has proven to be a promising technique for controlling cell properties such as adhesion or differentiation, resulting in enhanced osteointegration. However, the possibility of patterning the bone tissue side interface to generate microstructure effects has never been investigated. In the present study, three different laser-generated patterns were machined on the bone surface with the aim of identifying the best surface morphology compatible with osteogenic-related cell recolonization. The laser-patterned bone tissue was characterized by scanning electron microscopy and confocal microscopy in order to obtain a comprehensive picture of the bone surface morphology. The cortical bone patterning impact on cell compatibility and cytoskeleton rearrangement on the patterned surfaces was assessed using Stromal Cells from the Apical Papilla (SCAPs). The results indicated that laser machining had no detrimental effect on consecutively seeded cell metabolism. Orientation assays revealed that patterns with larger hatch distances were correlated with higher cell cytoskeletal conformation to the laser-machined patterns. To the best of our knowledge, this study is the first to consider and evaluate bone as a biological interface that can be engineered for improvement. Further investigations should focus on the in vivo implications of this direct patterning.
用于骨组织工程目的的植入材料激光图案化已被证明是一种很有前景的技术，可用于控制细胞特性，如粘附或分化，从而增强骨整合。然而，对骨组织侧界面进行图案化以产生微观结构效应的可能性从未被研究过。在本研究中，在骨表面加工了三种不同的激光产生的图案，目的是确定与成骨相关细胞重新定植相容的最佳表面形态。通过扫描电子显微镜和共聚焦显微镜对激光图案化的骨组织进行表征，以全面了解骨表面形态。使用根尖乳头间充质干细胞（SCAPs）评估皮质骨图案化对图案化表面上细胞相容性和细胞骨架重排的影响。结果表明，激光加工对连续接种的细胞代谢没有不利影响。取向分析表明，具有较大扫描间距的图案与细胞细胞骨架与激光加工图案的更高构象相关。据我们所知，本研究是首次将骨视为可进行工程改造以实现改善的生物界面。进一步的研究应集中在这种直接图案化的体内影响上。
