Tamegart Lahcen, Abbaoui Abdellatif, Makbal Rachida, Zroudi Mohamed, Bouizgarne Brahim, Bouyatas My Mustapha, Gamrani Halima
Cadi Ayyad University, Faculty of Sciences Semlalia, Neurosciences, Pharmacology and Environment Unit, Marrakesh, Morocco.
Cadi Ayyad University, Faculty of Sciences Semlalia, Marrakesh, Morocco.
Acta Histochem. 2019 Feb;121(2):171-181. doi: 10.1016/j.acthis.2018.12.003. Epub 2018 Dec 17.
Lead (Pb) is a metal element released into the atmosphere and a major source of environmental contamination. The accumulation and concentration of this metal in a food web may lead to the intoxication of the body, more precisely, the nervous system (NS). In addition, Pb-exposure can cause structural and functional disruption of the NS. Studies have shown that Pb-exposure could be a risk factor in the development of Parkinson's disease (PD). The latter is related to dopaminergic deficiency that may be triggered by genetic and environmental factors such as Pb intoxication. In this study, we have evaluated, in one hand, the neurotoxic effect of Pb (25 mg / kg B.W i.p) for three consecutive days on dopaminergic system and locomotor performance in Merione shawi. In the other hand, the possible restorative potential of C. sativus (CS) (50 mg / kg BW) by oral gavage. The immunohistochemical approach has revealed that Pb-intoxicated Meriones show a significant increase of Tyrosine Hydroxylase (TH) levels within the Substantia Nigra compacta (SNc), Ventral Tegmental Area (VTA), Locus Coeruleus (LC), Dorsal Striatum (DS) and Medial Forebrain Bundle (MFB), unlike the control meriones, a group intoxicated and treated with Crocus sativus hydroethanolic extract (CSHEE) and treated group by CSHEE. Treatment with CSHEE, has shown a real potential to prevent all Pb-induced damages. In fact, restores the TH levels by 92%, 90%, 88%, 90% and 93% in SNc, VTA, LC, DS and MFB respectively, similarly, locomotor activity dysfunction in Pb-intoxicaed meriones was reinstated by 90%. In this study, we have revealed a new pharmacological potential of Crocus sativus that can be used as a neuroprotective product for neurodegenerative disorders, especially, which implying dopaminergic and noradrenergic injuries, like PD, trigged by heavy metals.
铅（Pb）是一种释放到大气中的金属元素，也是环境污染的主要来源。这种金属在食物网中的积累和浓缩可能导致身体中毒，更确切地说是神经系统（NS）中毒。此外，铅暴露会导致神经系统的结构和功能破坏。研究表明，铅暴露可能是帕金森病（PD）发展的一个风险因素。后者与多巴胺能缺乏有关，而多巴胺能缺乏可能由遗传和环境因素如铅中毒引发。在本研究中，一方面，我们评估了连续三天腹腔注射25毫克/千克体重的铅对肖氏沙鼠多巴胺能系统和运动性能的神经毒性作用。另一方面，通过灌胃评估了藏红花（CS）（50毫克/千克体重）可能的恢复潜力。免疫组织化学方法显示，与对照组沙鼠、用藏红花水乙醇提取物（CSHEE）中毒并治疗的组以及CSHEE治疗组不同，铅中毒的沙鼠黑质致密部（SNc）、腹侧被盖区（VTA）、蓝斑（LC）、背侧纹状体（DS）和内侧前脑束（MFB）内酪氨酸羟化酶（TH）水平显著升高。用CSHEE治疗显示出预防所有铅诱导损伤的真正潜力。事实上，分别使SNc、VTA、LC、DS和MFB中的TH水平恢复了92%、90%、88%、90%和93%，同样，铅中毒沙鼠的运动活动功能障碍恢复了90%。在本研究中，我们揭示了藏红花的一种新的药理潜力，它可以用作神经退行性疾病的神经保护产品，特别是对于由重金属引发的涉及多巴胺能和去甲肾上腺素能损伤的疾病，如帕金森病。
