Gastroenterology Unit, University Campus Bio-Medico of Rome, Rome, Italy.
Human Microbiome Unit, Bambino Gesù Children's Hospital and Research Institute, Rome, Italy; Parasitology Unit, Bambino Gesù Children's Hospital and Research Institute, Rome, Italy.
Dig Liver Dis. 2019 May;51(5):648-656. doi: 10.1016/j.dld.2018.11.021. Epub 2018 Dec 3.
Growing evidence supports the potential role of intestinal microbiota in the pathophysiology of inflammatory bowel diseases (IBD) even if the literature does not reveal uniform alterations. The aim of the study was to evaluate the mucosal (MM) and faecal microbiota (FM) composition in a cohort of IBD patients compared to healthy controls (CTRLs).
Faecal and mucosal samples were collected from 14 IBD patients and 11 CTRLs. The V1-V3 region of 16S rRNA locus was amplified on a 454-Junior Genome Sequencer. Reads were grouped into operational taxonomic units (OTUs) at a sequence similarity level of 97% for taxonomic assignment, and aligned for OTUs matching against Greengenes database.
Irrespective of disease localization and activity, in the MM of IBD patients a statistically significant increase of Proteobacteria (especially Enterobacteriaceae, Acidaminococcus, Veillonella dispar) and decrease of Firmicutes (especially Roseburia and Faecalibacterium prausnitzii) and Actinobacteria was found compared to CTRLs. In the colon district some specific bacterial biomarkers were identified: Enterobacteriaceae for IBD stools, Bacteroides for IBD biopsies, Mogibacteriaceae, Ruminococcaceae and Prevotella for CTRL stools, Ruminococcaceae for CTRL biopsies.
The profiles of FM were more similar to CTRLs, suggesting that microbiota adhering to the gut mucosa better discriminates patients from controls, with the identification of some interesting biomarkers.
越来越多的证据支持肠道微生物群在炎症性肠病（IBD）的病理生理学中的潜在作用，尽管文献中并没有显示出一致的改变。本研究的目的是评估 IBD 患者的黏膜（MM）和粪便微生物群（FM）组成与健康对照（CTRLs）相比。
从 14 名 IBD 患者和 11 名 CTRLs 中收集粪便和黏膜样本。16S rRNA 基因座的 V1-V3 区在 454-Junior 基因组测序仪上扩增。将读取按序列相似性水平为 97%的操作分类单元（OTUs）分组，用于分类分配，并将 OTUs 与 Greengenes 数据库进行比对。
无论疾病的定位和活动如何，与 CTRLs 相比，IBD 患者的 MM 中 Proteobacteria（特别是 Enterobacteriaceae、Acidaminococcus、Veillonella dispar）的数量显著增加，Firmicutes（特别是 Roseburia 和 Faecalibacterium prausnitzii）和 Actinobacteria 的数量减少。在结肠区域，一些特定的细菌生物标志物被识别出来：IBD 粪便中的 Enterobacteriaceae，IBD 活检中的 Bacteroides，CTRL 粪便中的 Mogibacteriaceae、Ruminococcaceae 和 Prevotella，CTRL 活检中的 Ruminococcaceae。
FM 的特征与 CTRLs 更相似，这表明粘附在肠道黏膜上的微生物群能更好地区分患者和对照者，同时也鉴定出一些有趣的生物标志物。
