Chen Xiaojie, Xie Xuhong, Xing Yanfen, Yang Xiuhua, Yuan Zhaohu, Wei Yaming
Department of Blood Transfusion, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.
Guangdong Technology Engineering Center of Precision Blood Transfusion, Guangzhou, Guangdong, China.
Transfus Med Hemother. 2018 Nov;45(6):397-402. doi: 10.1159/000489321. Epub 2018 Aug 24.
Stored red blood cells (RBCs) undergo storage lesions involving morphological, physiological and biochemical changes. MicroRNAs (miRNAs) have important functions in cell apoptosis and life processes. Therefore, the aim of this study was to explore potential roles of miRNAs in the damage of stored RBCs.
Blood samples were collected from 13 healthy male O-type donors, and leuko-reduced RBCs were divided into fresh RBC group and 20-day storage RBC group.
Eight predicted miRNAs with modified expressions with an intersection ≥ 3 were found dysregulated in the 20-day storage RBC group and involved in apoptosis and senescence signaling pathway: miR-31-5p, miR-196a-5p, miR-203a, miR-654-3p and miR-769-3p were increased, while miR-96-5P, miR-150-5P and miR-197-3p were decreased. Evidence associating miR-31-5p, miR-203a, miR-654 and miR-769 to RBCs or blood in general are not available.
Dysregulated miRNAs might represent potential biomarkers to identify storage lesions, and their detection might help to evaluate the quality of stored RBCs.
储存的红细胞（RBCs）会经历包括形态、生理和生化变化在内的储存损伤。微小RNA（miRNAs）在细胞凋亡和生命过程中具有重要功能。因此，本研究旨在探讨miRNAs在储存红细胞损伤中的潜在作用。
从13名健康男性O型献血者采集血样，将去白细胞的红细胞分为新鲜红细胞组和储存20天的红细胞组。
在储存20天的红细胞组中发现8种预测的miRNAs表达改变，交集≥3，且参与凋亡和衰老信号通路：miR-31-5p、miR-196a-5p、miR-203a、miR-654-3p和miR-769-3p升高，而miR-96-5P、miR-150-5P和miR-197-3p降低。目前尚无miR-31-5p、miR-203a、miR-654和miR-769与红细胞或一般血液相关的证据。
失调的miRNAs可能是识别储存损伤的潜在生物标志物，检测它们可能有助于评估储存红细胞的质量。
