miR-20a-5p 的上调可作为红细胞储存损伤的潜在 miRNA 标志物。
Upregulation of miR-20a-5p as the Potential MicroRNA Marker in Red Blood Cell Storage Lesion.
机构信息
Medical Technology, School of Allied Health Sciences, Walailak University, Tha Sala, Nakhon Si Thammarat, Thailand.
Hematology and Transfusion Science Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.
出版信息
Biomed Res Int. 2023 Oct 4;2023:5598590. doi: 10.1155/2023/5598590. eCollection 2023.
BACKGROUND
Packed red blood cells (PRBCs) can be preserved for 42 days, and stored PRBCs have slow, dangerous changes over time during storage. miRNA is approximately 22 nucleotides long, a small single-stranded noncoding RNA molecule. miRNA guides by pairing bases with their downstream target mRNA to regulate negative expression. They are essential in many life processes, including cell differentiation, proliferation, and apoptosis. Therefore, miRNA alterations may represent possible biomarkers of PRBC storage lesions. This study is aimed at validating the miR-20a-5p in PRBC storage. . A total of 20 PRBC samples were divided into day 1 and day 20 storage groups. Total miRNA was extracted and quantified by probe-based RT-qPCR assays to explore the potential role of miRNAs in PRBC storage lesions.
RESULTS
Upregulated miR-20a-5p in PRBC storage on day 20 compared to day 1. MiR-20a-5p promoted cell survival, which may affect the downstream regulation and decrease PRBC viability in prolonged storage.
CONCLUSION
On this basis, this detection may help to assess the quality of stored PRBCs.
背景
浓缩红细胞（PRBC）可保存 42 天，在储存过程中，储存的 PRBC 会随着时间的推移发生缓慢而危险的变化。miRNA 约 22 个核苷酸长，是一种小的单链非编码 RNA 分子。miRNA 通过与下游靶 mRNA 的碱基配对来指导，从而调节负表达。它们在许多生命过程中至关重要，包括细胞分化、增殖和凋亡。因此，miRNA 的改变可能代表 PRBC 储存损伤的潜在生物标志物。本研究旨在验证 PRBC 储存中的 miR-20a-5p。总共 20 个 PRBC 样本分为第 1 天和第 20 天储存组。通过基于探针的 RT-qPCR 检测提取和定量总 miRNA，以探讨 miRNAs 在 PRBC 储存损伤中的潜在作用。
结果
与第 1 天相比，第 20 天 PRBC 储存中的 miR-20a-5p 上调。miR-20a-5p 促进细胞存活，这可能会影响下游调节并降低 PRBC 在长时间储存中的活力。
结论
在此基础上，这种检测可能有助于评估储存的 PRBC 的质量。
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