School of Basic Medical Sciences of Southwest Medical University, Luzhou, Sichuan, China.
College of Life Science, University of the Chinese Academy of Sciences, Beijing, China.
Life Sci. 2019 Feb 1;218:58-64. doi: 10.1016/j.lfs.2018.12.032. Epub 2018 Dec 19.
The purpose of this work was to study the effects and underlying molecular mechanisms of ligustilide on cancer-associated fibroblasts (CAFs). The effects of ligustilide on the growth of CAFs and splenocytes were detected by MTT assay, and flow cytometry was used to detect effects on T-cell proliferation. Western blotting was used to detect the expression levels of CAF-related proteins after ligustilide treatment. This study found that ligustilide had no effect on the growth of splenocytes but that it could change the immunosuppressive function of CAFs through the TLR4-NF-κB pathway and restore T-cell proliferation previously inhibited by the CAF supernatant. Thus, ligustilide is expected to be a candidate for new antitumor drugs.
本工作旨在研究蛇床子素对癌相关成纤维细胞（CAFs）的作用及其潜在分子机制。通过 MTT 法检测蛇床子素对 CAFs 和脾细胞生长的影响，流式细胞术检测对 T 细胞增殖的影响。Western blot 检测蛇床子素处理后 CAF 相关蛋白的表达水平。本研究发现，蛇床子素对脾细胞的生长无影响，但能通过 TLR4-NF-κB 通路改变 CAF 的免疫抑制功能，恢复 CAF 上清液抑制的 T 细胞增殖。因此，蛇床子素有望成为新型抗肿瘤药物的候选药物。
