川芎内酯通过 TLR4 通路促进癌相关成纤维细胞凋亡。
Ligustilide promotes apoptosis of cancer-associated fibroblasts via the TLR4 pathways.
机构信息
Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China; Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; School of Basic Medical Sciences of Southwest Medical University, Luzhou, Sichuan, China.
Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
出版信息
Food Chem Toxicol. 2020 Jan;135:110991. doi: 10.1016/j.fct.2019.110991. Epub 2019 Nov 22.
The goal of this research was to study the selective pro-apoptotic effect of ligustilide on prostate-cancer-associated fibroblast in the tumor microenvironment and the related molecular mechanisms. The effects of ligustilide on cancer-associated fibroblasts (CAFs) and normal fibroblasts (NFs) isolated from the prostate were determined by MTT assay. Flow cytometry and cellular immunofluorescence were used to detect the effects of ligustilide on the cell cycle and apoptosis. Western blotting was used to detect the expression of apoptosis-related proteins after the action of ligustilide on CAFs. In the investigation, ligustilide had a selective pro-apoptotic effect on prostate-CAFs. After ligustilide treatment, the proportion of CAFs in the G2-M phase of the cell cycle increased, and the expression of apoptosis-related proteins (p-P53, Bcl-2, Caspase9 and Cytochrome C) changed. Ligustilide blocks the CAF cell cycle and induces the apoptosis of CAFs.
本研究旨在研究蛇床子素在肿瘤微环境中对前列腺癌相关成纤维细胞的选择性促凋亡作用及其相关分子机制。通过 MTT 法测定蛇床子素对前列腺分离的癌相关成纤维细胞（CAFs）和正常成纤维细胞（NFs）的影响。流式细胞术和细胞免疫荧光检测蛇床子素对细胞周期和细胞凋亡的影响。Western blot 检测蛇床子素作用于 CAFs 后凋亡相关蛋白的表达。在研究中，蛇床子素对前列腺-CAFs 具有选择性促凋亡作用。蛇床子素处理后，CAFs 细胞周期 G2-M 期比例增加，凋亡相关蛋白（p-P53、Bcl-2、Caspase9 和 Cytochrome C）表达发生变化。蛇床子素阻断 CAF 细胞周期并诱导 CAF 凋亡。
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