Sekiguchi S, Ikeda H, Kato T, Maeda R, Fukai K
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Transfusion. 1988 Nov-Dec;28(6):581-5. doi: 10.1046/j.1537-2995.1988.28689059036.x.
An automated pretransfusion testing system was applied to HIV antibody screening by a gelatin particle agglutination (HIV-PA) test. The test conditions for the test, such as diluent, plate shape, and incubation time, were applicable not only for the HIV-PA test but also for other routine tests, including those for hepatitis B surface antigen and HTLV-1 antibodies. After a 60-minute incubation, the plates were read automatically and then were assessed visually. Tests with seropositive samples obtained from hemophiliacs and an HIV enzyme immunoassay (EIA) familiarization panel showed that the automated HIV-PA test was more sensitive than the EIA and did not show a false-negative result. Of 11,300 blood donors, 44 were positive by the automated test, and 51 were positive by the EIA. None of the blood donors was confirmed to be seropositive. Using the automated test, a large number of samples could be screened in a relatively short time without a significant increase of nonspecific reactions.
一种自动输血前检测系统应用于通过明胶颗粒凝集试验（HIV-PA）进行HIV抗体筛查。该检测的试验条件，如稀释剂、平板形状和孵育时间，不仅适用于HIV-PA检测，也适用于其他常规检测，包括乙肝表面抗原和HTLV-1抗体检测。孵育60分钟后，自动读取平板，然后进行目视评估。对从血友病患者获得的血清阳性样本和一个HIV酶免疫测定（EIA）熟悉样本组进行的检测表明，自动HIV-PA检测比EIA更敏感，且未出现假阴性结果。在11300名献血者中，自动检测有44例呈阳性，EIA检测有51例呈阳性。没有献血者被确认为血清阳性。使用自动检测，可以在相对较短的时间内筛查大量样本，且非特异性反应没有显著增加。
