Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu 610059, China.
School of Food and Biological Engineering, Xihua University, Chengdu 610039, China.
Int J Environ Res Public Health. 2018 Dec 20;16(1):1. doi: 10.3390/ijerph16010001.
Quinoprotein glucose dehydrogenase (GDH) is the most important enzyme of inorganic phosphorus-dissolving metabolism, catalyzing the oxidation of glucose to gluconic acid. The insoluble phosphate in the sediment is converted into soluble phosphate, facilitating mass reproduction of algae. Therefore, studying the diversity of genes which encode GDH is beneficial to reveal the microbial group that has a significant influence on the eutrophication of water. Taking the eutrophic Sancha Lake sediments as the research object, we acquired samples from six sites in the spring and autumn. A total of 219,778 high-quality sequences were obtained by DNA extraction of microbial groups in sediments, PCR amplification of the gene, and high-throughput sequencing. Six phyla, nine classes, 15 orders, 29 families, 46 genera, and 610 operational taxonomic units (OTUs) were determined, suggesting the high genetic diversity of . genes came mainly from the genera of (1.63⁻77.99%), (0.13⁻56.95%), (0.32⁻25.49%), and (0.16⁻11.88%) in the phylum of Proteobacteria (25.10⁻98.85%). The abundance of these dominant -harboring bacteria was higher in the spring than in autumn, suggesting that they have an important effect on the eutrophication of the Sancha Lake. The alpha and beta diversity of genes presented spatial and temporal differences due to different sampling site types and sampling seasons. Pearson correlation analysis and canonical correlation analysis (CCA) showed that the diversity and abundance of genes were significantly correlated with environmental factors such as dissolved oxygen (DO), phosphorus hydrochloride (HCl⁻P), and dissolved total phosphorus (DTP). OTU composition was significantly correlated with DO, total organic carbon (TOC), and DTP. GDH encoded by genes transformed insoluble phosphate into dissolved phosphate, resulting in the eutrophication of Sancha Lake. The results suggest that genes encoding GDH may play an important role in lake eutrophication.
醌蛋白葡萄糖脱氢酶（GDH）是无机磷溶解代谢中最重要的酶，它催化葡萄糖氧化为葡萄糖酸。沉积物中的不溶性磷酸盐被转化为可溶性磷酸盐，促进藻类的大量繁殖。因此，研究编码 GDH 的基因多样性有助于揭示对水体富营养化有显著影响的微生物群。以富营养化的三岔湖沉积物为研究对象，我们在春季和秋季从六个地点采集样品。通过从沉积物中提取微生物群体的 DNA、PCR 扩增基因和高通量测序，共获得了 219778 条高质量的序列。确定了六个门、九个纲、十五个目、二十九个科、四十六个属和 610 个操作分类单元（OTU），表明 GDH 基因的遗传多样性很高，主要来源于变形菌门的（1.63⁻77.99%）、酸杆菌门（0.13⁻56.95%）、放线菌门（0.32⁻25.49%）和拟杆菌门（0.16⁻11.88%）的（0.13⁻56.95%）。这些优势 - 携带细菌的丰度在春季高于秋季，表明它们对三岔湖的富营养化有重要影响。由于采样地点类型和采样季节的不同，基因的α和β多样性呈现出时空差异。Pearson 相关分析和典范对应分析（CCA）表明，基因的多样性和丰度与溶解氧（DO）、盐酸盐（HCl⁻P）和溶解总磷（DTP）等环境因素显著相关。OTU 组成与 DO、总有机碳（TOC）和 DTP 显著相关。编码 GDH 的基因将不溶性磷酸盐转化为可溶性磷酸盐，导致三岔湖富营养化。结果表明，编码 GDH 的基因可能在湖泊富营养化中发挥重要作用。
