Zhao Xingxing, Zhang Ying, Cui Zhenbo, Peng Lu, Cao Chengyou
College of Life and Health Sciences, Northeastern University, Shenyang, China.
Front Microbiol. 2022 Mar 4;13:831888. doi: 10.3389/fmicb.2022.831888. eCollection 2022.
Biological soil crusts (BSCs) are important for restoring vegetation and improving soil fertility in arid or semiarid desertified land. However, studies on the contribution of BSC microbes to phosphorus (P) transformation remains limited. The microbial diversity involved in P transformation and its dynamic along BSC development should be examined to further understand the microbial regulatory mechanism of the P-cycling process. This paper investigates the soil properties, P fractions, and potential of P transformation across a chronosequence (0-, 8-, 20-, and 35-year) of the BSC under plantation on the moving sand dunes in Horqin Grassland, China. An abundance of and genes was detected, and the diversities and structures of - and -haboring microbial communities were illustrated high-throughput sequencing. Soil nutrient content, activity of alkaline phosphomonoesterase, potential of organic P (OP) mineralization, and the abundance of and genes all linearly increased along with BSC age. The microbial quantity and species diversity of the community were greater than those of . BSC development increased the availability of inorganic P (IP) fractions, and both NaHCO-Pi and NaOH-Pi were positively correlated with the abundance of the two genes and the activity of alkaline phosphomonoesterase. The phyla of Actinobacteria, Planctomycetes, and Proteobacteria and the family of were the most dominant taxa in the community, Proteobacteria was the dominant phylum in the community in BSC soils, and and were the most dominant genera. The dominant taxa quantitatively responded to soil property improvement, but the basic compositions and dominant taxa did not change along with BSC development. The structures of and communities were linked to soil properties, and pH available K, and total K tend to be the direct determining factors.
生物土壤结皮（BSCs）对于干旱或半干旱荒漠化土地的植被恢复和土壤肥力改善具有重要意义。然而，关于BSC微生物对磷（P）转化的贡献的研究仍然有限。为了进一步了解P循环过程的微生物调节机制，应研究参与P转化的微生物多样性及其随BSC发育的动态变化。本文研究了中国科尔沁草原流动沙丘上人工种植条件下BSC不同年代序列（0年、8年、20年和35年）的土壤性质、P形态以及P转化潜力。通过高通量测序检测到大量的[具体基因名称1]和[具体基因名称2]基因，并阐明了携带[具体基因名称1]和[具体基因名称2]的微生物群落的多样性和结构。土壤养分含量、碱性磷酸单酯酶活性、有机P（OP）矿化潜力以及[具体基因名称1]和[具体基因名称2]基因的丰度均随BSC年龄呈线性增加。[具体微生物群落1]的微生物数量和物种多样性大于[具体微生物群落2]。BSC发育增加了无机P（IP）形态的有效性，NaHCO-Pi和NaOH-Pi均与两个基因的丰度以及碱性磷酸单酯酶活性呈正相关。放线菌门、浮霉菌门和变形菌门以及[具体科名称]是[具体微生物群落1]中最主要的分类群，变形菌门是BSC土壤中[具体微生物群落2]的优势门，[具体属名称1]和[具体属名称2]是最主要的属。优势分类群对土壤性质改善有定量响应，但基本组成和优势分类群并未随BSC发育而改变。[具体微生物群落1]和[具体微生物群落2]的结构与土壤性质相关，pH、有效K和全K往往是直接决定因素。
